International Journal of Legal Medicine

, Volume 130, Issue 5, pp 1191–1194 | Cite as

Analysis of 27 Y-chromosomal STR haplotypes in a Han population of Henan province, Central China

Population Data

Abstract

A total of 1225 unrelated Han males from Henan province were analyzed with the prototype Yfiler® Plus kit (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA). The calculated gene diversity (GD) values ranged from 0.3855 to 0.9673 for the DYS391 and DYS385a/b loci, respectively. The discriminatory capacity (DC) was 86.94 % with 1065 observed haplotypes using 17 Yfiler loci, by the addition of 10 Y-STRs to the Yfiler® Plus system, the DC was increased to 98.94 % while showing 1212 observed haplotypes. Among the new incorporated Y-STRs, DYS576, DYF387S1, DYS518, DYS627, and DYS449 were major contributors to enhancing discrimination. In the analysis of molecular variance, the Henan Han population clustered with Asian origin populations and showed significant differences from other reference populations. In this study, the improvement of adding additional Y-STR markers with the Yfiler® Plus kit provided substantially stronger discriminatory power in the Henan Han population.

Keywords

Y-STR Yfiler®Plus Haplotypes Multidimensional scaling Henan Han 

Supplementary material

414_2016_1326_MOESM1_ESM.xls (340 kb)
ESM 1(XLS 340 kb)
414_2016_1326_MOESM2_ESM.docx (20 kb)
ESM 2(DOCX 19 kb)

References

  1. 1.
    Roewer L (2009) Y chromosome STR typing in crime casework. Forensic Sci Med Pathol 5:77–84CrossRefPubMedGoogle Scholar
  2. 2.
    de Knijff P, Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, Heidorn F, Herrmann S, Herzog B, Hidding M, Honda K, Jobling M, Krawczak M, Leim K, Meuser S, Meyer E, Oesterreich W, Pandya A, Parson W, Penacino G, Perez-Lezaun A, Piccinini A, Prinz M, Roewer L (1997) Chromosome Y microsatellites: population genetic and evolutionary aspects. Int J Legal Med 110:134–149CrossRefPubMedGoogle Scholar
  3. 3.
    Hall A, Ballantyne J (2003) Novel Y-STR typing strategies reveal the genetic profile of the semen donor in extended interval postcoital cervico vaginal samples. Forensic Sci Int 136:58–72CrossRefPubMedGoogle Scholar
  4. 4.
    Thompson JM, Ewing MM, Frank WE, Pogemiller JJ, Nolde CA, Koehler DJ, Shaffer AM, Rabbach DR, Fulmer PM, Sprecher CJ, Storts DR (2013) Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples. Forensic Sci Int Genet 7:240–250CrossRefPubMedGoogle Scholar
  5. 5.
    Ballantyne KN, Keerl V, Wollstein A, Choi Y, Zuniga SB, Ralf A, Vermeulen M, de Knijff P, Kayser M (2012) A new future of forensic Y-chromosome analysis: rapidly mutating Y-STRs for differentiating male relatives and paternal lineages. Forensic Sci Int Genet 6:208–218CrossRefPubMedGoogle Scholar
  6. 6.
    Shi MS, Liu YJ, Zhang JT, Bai RF, Lv XJ, Ma SH (2015) Analysis of 24 Y chromosomal STR haplotypes in a Chinese Han population sample from Henan Province, Central China. Forensic Sci Int Genet 17:83–86CrossRefPubMedGoogle Scholar
  7. 7.
    Willuweit S, Roewer L (2015) The new Y chromosome haplotype reference database. Forensic Sci Int Genet 15:43–48CrossRefPubMedGoogle Scholar
  8. 8.
    Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York, pp 176–179Google Scholar
  9. 9.
    Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567CrossRefPubMedGoogle Scholar
  10. 10.
    Enrica O, Stefano V, Paola A, Alessandro A, Paola M (2015) Preliminary assessment of the prototype Yfiler® Plus kit in a population study of Northern Italian males. Int J Legal Med 129:729–730CrossRefGoogle Scholar
  11. 11.
    Olofsson JK, Mogensen HS, Buchard A, Børsting C, Morling N (2015) Forensic and population analyses of Danes, Greenlanders and Somalis typed with the Yfiler® Plus PCR amplification kit. Forensic Sci Int Genet 16:232–236CrossRefPubMedGoogle Scholar
  12. 12.
    García O, Yurrebaso I, Mancisidor ID, López S, Alonso S, Gusmão L (2015) Data for 27 Y-chromosome STR loci in the Basque Country autochthonous population. Forensic Sci Int Genet. doi:10.1016/j.fsigen.2015.09.010 Google Scholar
  13. 13.
    Parson W, Roewer L (2010) Publication of population data of linearly inherited DNA markers in the International Journal of Legal Medicine. Int J Legal Med 124:505–509CrossRefPubMedGoogle Scholar
  14. 14.
    Poetsch M, Bajanowski T, Pfeiffer H (2012) The publication of population genetic data in the International Journal of Legal Medicine: guidelines. Int J Legal Med 126:489–490CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Rufeng Bai
    • 1
    • 2
  • Yaju Liu
    • 3
  • Juntao Zhang
    • 3
  • Meisen Shi
    • 1
    • 2
  • Hongmei Dong
    • 4
  • Shuhua Ma
    • 5
  • R. F. Bai
    • 1
    • 2
  • MS. Shi
    • 1
    • 2
  1. 1.Key Laboratory of Evidence Science (China University of Political Science and Law)Ministry of EducationBeijingPeople’s Republic of China
  2. 2.Collaborative Innovation Center of Judicial CivilizationBeijingPeople’s Republic of China
  3. 3.Xuchang Institute of Forensic SciencesPublic Security Bureau of Henan ProvinceHenanPeople’s Republic of China
  4. 4.Department of Forensic MedicineHuazhong University of Science and Technology, Tongji Medical CollegeHubeiPeople’s Republic of China
  5. 5.Department of Radiology, First Affiliated HospitalMedical College of Shantou UniversityShantouPeople’s Republic of China

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