Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China


The six-dye GlobalFiler™ Express PCR amplification kit incorporates 21 commonly used autosomal short tandem repeat (STR) loci and three gender determination loci. In this study, we analyzed the GlobalFiler STR loci on 748 unrelated individuals from a Chinese Kazakh population of Xinjiang, China. No significant deviations from Hardy-Weinberg equilibrium and linkage disequilibrium were observed within and between 21 autosomal STR loci. SE33 showed the greatest power of discrimination in Kazakh population. The combined power of discrimination of Kazakh was 99.999999999999999999999996797 %. No significant differences of allele frequencies were observed between Kazakh and Uyghur at all 15 tested STR loci, as well as Mongolian. Significant differences were only observed between Kazakh and the other Chinese populations at TH01. Multiple STR loci showed significant differences between Kazakh and Arab, as well as South Portuguese. The multidimensional scaling plot (MDS) plot and neighbor-joining tree also showed Kazakh is genetically close to Uyghur.

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  1. 1.

    Hammond HA, Jin L, Zhong Y, Caskey CT, Chakraborty R (1994) Evaluation of 13 short tandem repeat loci for use in personal identification applications. Am J Hum Genet 55(1):175–189

    CAS  PubMed  PubMed Central  Google Scholar 

  2. 2.

    Gettings KB, Aponte RA, Vallone PM, Butler JM (2015) STR allele sequence variation: current knowledge and future issues. Forensic Sci Int-Genet 18:118–130. doi:10.1016/j.fsigen.2015.06.005

    CAS  Article  Google Scholar 

  3. 3.

    Wang DY, Gopinath S, Lagacé RE, Norona W, Hennessy LK, Short ML, Mulero JJ (2015) Developmental validation of the GlobalFiler® Express PCR Amplification Kit: a 6-dye multiplex assay for the direct amplification of reference samples. Forensic Sci Int-Genet 19:148–155. doi:10.1016/j.fsigen.2015.07.013

    CAS  Article  Google Scholar 

  4. 4.

    Flores S, Sun J, King J, Budowle B (2014) Internal validation of the GlobalFiler (TM) Express PCR Amplification Kit for the direct amplification of reference DNA samples on a high-throughput automated workflow. Forensic Sci Int-Genet 10:33–39. doi:10.1016/j.fsigen.2014.01.005

    CAS  Article  Google Scholar 

  5. 5.

    Martin P, de Simon LF, Luque G, Farfan MJ, Alonso A (2014) Improving DNA data exchange: validation studies on a single 6 dye STR kit with 24 loci. Forensic Sci Int-Genet 13:68–78. doi:10.1016/j.fsigen.2014.07.002

    CAS  Article  Google Scholar 

  6. 6.

    Almeida C, Ribeiro T, Oliveira AR, Porto MJ, Santos JC, Dias D, Dario P (2015) Population data of the GlobalFiler® Express loci in South Portuguese population. Forensic Sci Int-Genet 19:39–41. doi:10.1016/j.fsigen.2015.06.001

    CAS  Article  Google Scholar 

  7. 7.

    Alhmoudi OA, Jones RJ, Tay GK, Alsafar H, Hadi S (2015) Population genetics data for 21 autosomal STR loci for United Arab Emirates (UAE) population using next generation multiplex STR kit. Forensic Sci Int-Genet 19:190–191. doi:10.1016/j.fsigen.2015.07.009

    Article  Google Scholar 

  8. 8.

    Yuan JY, Wang XY, Shen CM, Liu WJ, Yan JW, Wang HD, Pu HW, Wang YL, Yang G, Zhang YD, Meng HT, Jing H, Zhu BF (2014) Genetic profile characterization and population study of 21 autosomal STR in Chinese Kazak ethnic minority group. Electrophoresis 35(4):503–510. doi:10.1002/elps.201300398

    CAS  Article  Google Scholar 

  9. 9.

    Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr W, Olaisen B (1997) DNA recommendations—further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. Int J Legal Med 110(4):175–176. doi:10.1007/s004140050061

    CAS  Article  Google Scholar 

  10. 10.

    Olaisen B, Bar W, Brinkmann B, Budowle B, Carracedo A, Gill P, Lincoln P, Mayr WR, Rand S (1998) DNA recommendations 1997 of the International Society for Forensic Genetics. Vox Sang 74(1):61–63. doi:10.1046/j.1423-0410.1998.7410061.x

    CAS  Article  Google Scholar 

  11. 11.

    Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567. doi:10.1111/j.1755-0998.2010.02847.x

    Article  Google Scholar 

  12. 12.

    Zhao F, Wu X, Cai G, Xu C (2003) The application of Modified-Powerstates software in forensic biostatistics (in Chinese). Chinese Journal of Forensic Medicine 18(5):297–298

    Google Scholar 

  13. 13.

    Weir BS, Hill WG (2002) Estimating F-statistics. Annual Review of Genetics 36:721–750

    CAS  Article  Google Scholar 

  14. 14.

    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    CAS  Article  Google Scholar 

  15. 15.

    Chakraborty R, Kidd KK (1991) The utility of DNA typing in forensic work. Science 254(5039):1735–1739. doi:10.1126/science.1763323

    CAS  Article  Google Scholar 

  16. 16.

    Li L, Xu J, Liu X, Chen W, Xia M, Yang S, Jiang P, Ma T, Yang Y, Qian J, Sun H, Hu R, Miqin, Feng Z, Zuo Y, Zhou R, Ping Y, Zhou H, Zhao Z, Jin L, Li S (2015) Population data of 15 short tandem repeat loci in 1084 individuals from six Han and four ethnic populations in China. Forensic Sci Int-Genet 19:146–147. doi:10.1016/j.fsigen.2015.06.015

  17. 17.

    Lou HY, Li SL, Jin WF, Fu RQ, Lu DS, Pan XW, Zhou HG, Ping Y, Jin L, Xu SH (2015) Copy number variations and genetic admixtures in three Xinjiang ethnic minority groups. Eur J Hum Genet 23(4):536–542. doi:10.1038/ejhg.2014.134

    CAS  Article  Google Scholar 

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This study was supported by grants from the National Science Foundation of China (31271338), Project of Chinese Ministry of Education (113022A) and the National High Technology Research and Development Program (2012AA021802).

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Correspondence to Liming Li or Shilin Li.

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The authors declare that they have no competing interests.

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Honghua Zhang and Shuping Yang contributed equally to this work.

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Zhang, H., Yang, S., Guo, W. et al. Population genetic analysis of the GlobalFiler STR loci in 748 individuals from the Kazakh population of Xinjiang in northwest China. Int J Legal Med 130, 1187–1189 (2016). https://doi.org/10.1007/s00414-016-1319-2

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  • Short tandem repeat
  • GlobalFiler
  • Kazakh
  • Xinjiang