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International Journal of Legal Medicine

, Volume 132, Issue 4, pp 1231–1239 | Cite as

The contribution of Micro-CT to the evaluation of trabecular bone at the posterior part of the auricular surface in men

  • Céline Deguette
  • Daniel Chappard
  • Hélène Libouban
  • Guillaume Airagnes
  • Clotilde Rouge-Maillart
  • Norbert Telmon
Technical Note

Abstract

Aim

Using multi-slice computed tomography (MSCT), Barrier et al. described the disappearance at the posterior auricular surface of a “central line” (CL) and “juxtalinear cells” (JLCs) belonging to a trabecular bundle, and a trabecular density gradient around the CL that decreased with age. The aim of our study was to use micro-CT to test these findings, referring to the concept of Ascadi and Nemeskeri.

Methodology

The coxal bones of fifteen males were used; age was known. CLs were identified on MSCT-sections using Barrier’s method (64 detectors, 0.6 mm slice thickness, 0.1 mm overlap) with two different software programs (Synapse®, Amira®). Then, CLs were researched on microCT slices (pixel size: 36 μm). Three volumes of interest were defined (around, above, and below CL), and 3D morphometric parameters of the trabecular microarchitecture (particularly BV/TV and DA) were calculated. Two-tailed statistical analyses were performed attempting to correlate these parameters with age at death.

Results

CLs and JLCs were observed on micro-CT slices, but with moderate agreement between both imaging techniques. Their presence was not correlated with the age of the subjects. Around the CL, BV/TV decreased significantly with age; DA was negatively correlated with BV/TV and had a tendency to increase with age. Between areas above and below the CL, there was a BV/TV gradient and both BV/TVs decreased in parallel with age.

Conclusion

Our findings regarding the contribution of micro-CT to the evaluation of trabecular bone could be a promising research approach for application in a larger study population.

Keywords

Forensic science Forensic anthropology Trabecular bone Age at death MSCT Micro-CT Auricular surface 

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Céline Deguette
    • 1
    • 2
  • Daniel Chappard
    • 2
  • Hélène Libouban
    • 2
  • Guillaume Airagnes
    • 3
    • 6
  • Clotilde Rouge-Maillart
    • 1
    • 2
  • Norbert Telmon
    • 4
    • 5
  1. 1.CHU Angers, Department of Forensic MedicineLUNAM UniversitéAngers CedexFrance
  2. 2.GEROM Groupe Etudes Remodelage Osseux et bioMatériauxLUNAM Université, IRIS-IBS Institut de Biologie en Santé, CHU d’AngersAngers CedexFrance
  3. 3.AP-HP, Department of PsychiatryHôpitaux Universitaires Paris OuestParisFrance
  4. 4.Laboratoire Anthropologie Moléculaire et Imagerie de synthèseUniversité Paul SabatierToulouse CedexFrance
  5. 5.Department of Forensic MedicineCHU Toulouse RangueilToulouse CedexFrance
  6. 6.Faculté de MédecineUniversité Paris Descartes, Sorbonne Paris CitéParisFrance

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