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Genetic variation of 12 X-chromosomal STR loci in an East Timor sample

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Abstract

East Timor (República Democrática de Timor Leste) is a country with a population around 1 million inhabitants located in Southeast Asia and composed of 13 districts, including the eastern half of the Timor Island, Ataúro and Jaco islands, and the coastal enclave of Oecusse-Ambeno located in West Timor. Examples of the importance of X-chromosomal short tandem repeats (STRs) analysis include parentage identification, namely in cases involving father/daughter relationships, paternity in close relative deficiency cases without access to the putative father, maternity testing, and in rape or incest cases. In this study, 149 saliva samples were collected from unrelated individuals from East Timor and 12 X-chromosomal STRs genotyped using Investigator® Argus X-12 kit (Qiagen). A total of 13 alleles not included in Investigator Argus X-12 allelic ladder (off-ladder alleles) were found, four of which never reported (alleles 34.1 and 38.1 at DXS10134, allele 17.2 at DXS10074, and allele 28.1 at DXS10146). Allele 27.3 at DXS10101 and alleles 26, 28, and 29 at DXS10148 have already been observed in other populations but their frequencies are considerably higher in East Timor population. Allele frequencies and population statistic parameters were calculated for East Timor population and data contextualized in Southeast Asia/Pacific Region.

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Acknowledgments

The authors would like to thank all volunteer donors, the government of East Timor, the Universidade Nacional de Timor Lorosae (UNTL), the QIAGEN, and the Fundação para a Ciência e a Tecnologia (FCT), for their support and cooperation. This research was supported by the FCT Project: PTDC/CS-ANT/108558/2008, Programa Operacional Temático, Fatores de Competitividade (COMPETE), Quadro Comunitário de Apoio III and FEDER.

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Authors and Affiliations

  1. Department of Biology, University of Aveiro, Aveiro, Portugal

    Helena Moreira, Helena Costa, Filipa Tavares & Luís Souto

  2. Aveiro Institute for Biomedicine, iBiMED & Biology Department, University of Aveiro, Aveiro, Portugal

    Luís Souto

Authors
  1. Helena Moreira
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  2. Helena Costa
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  3. Filipa Tavares
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  4. Luís Souto
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Corresponding author

Correspondence to Luís Souto.

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Moreira, H., Costa, H., Tavares, F. et al. Genetic variation of 12 X-chromosomal STR loci in an East Timor sample. Int J Legal Med 129, 257–258 (2015). https://doi.org/10.1007/s00414-014-1126-6

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  • DOI: https://doi.org/10.1007/s00414-014-1126-6

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