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Development of a forensic identity SNP panel for Indonesia

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Abstract

Genetic markers included in forensic identity panels must exhibit Hardy-Weinberg and linkage equilibrium (HWE and LE). “Universal” panels designed for global use can fail these tests in regional jurisdictions exhibiting high levels of genetic differentiation such as the Indonesian archipelago. This is especially the case where a single DNA database is required for allele frequency estimates to calculate random match probabilities (RMPs) and associated likelihood ratios (LRs). A panel of 65 single nucleotide polymorphisms (SNPs) and a reduced set of 52 SNPs have been selected from 15 Indonesian subpopulations in the HUGO Pan Asian SNP database using a SNP selection strategy that could be applied to any panel of forensic identity markers. The strategy consists of four screening steps: (1) application of a G test for HWE; (2) ranking for high heterozygosity; (3) selection for LE; and (4) selection for low inbreeding depression. SNPs in our Indonesian panel perform well in comparison to some other universal SNP and short tandem repeat (STR) panels as measured by Fisher’s exact test for HWE and LE and Wright’s F statistics.

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Ethical standards

The experiments in this study comply with the current laws of Australia and Indonesia.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Forensic DNA Laboratory, Police Medicine Centre, Indonesian National Police, Jakarta, Indonesia

    Daniel Augustinus

  2. National Centre for Forensic Studies, University of Canberra, Bruce, ACT, Australia

    Daniel Augustinus, Michelle E. Gahan & Dennis McNevin

Authors
  1. Daniel Augustinus
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  2. Michelle E. Gahan
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  3. Dennis McNevin
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Corresponding author

Correspondence to Dennis McNevin.

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Augustinus, D., Gahan, M.E. & McNevin, D. Development of a forensic identity SNP panel for Indonesia. Int J Legal Med 129, 681–691 (2015). https://doi.org/10.1007/s00414-014-1055-4

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  • DOI: https://doi.org/10.1007/s00414-014-1055-4

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