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International Journal of Legal Medicine

, Volume 128, Issue 6, pp 923–931 | Cite as

Identification of human DNA in forensic evidence by loop-mediated isothermal amplification combined with a colorimetric gold nanoparticle hybridization probe

  • Khanistha Watthanapanpituck
  • Wansika Kiatpathomchai
  • Eric Chu
  • Nathinee PanvisavasEmail author
Original Article

Abstract

A DNA test based on loop-mediated isothermal amplification (LAMP) and colorimetric gold nanoparticle (AuNP) hybridization probe to detect the presence of human DNA in forensic evidence was developed. The LAMP primer set targeted eight regions of the human cytochrome b, and its specificity was verified against the DNA of 11 animal species, which included animals closely related to humans, such as chimpanzee and orangutan. By using the AuNP probe, sequence-specific LAMP product could be detected and the test result could be visualized through the change in color. The limit of detection was demonstrated with reproducibility to be as low as 718 fg of genomic DNA, which is equivalent to approximately 100 plasmid DNA copies containing the cytochrome b DNA target region. A simple DNA extraction method for the commonly found forensic biological samples was also devised to streamline the test process. This LAMP-AuNP human DNA test showed to be a robust, specific, and cost-effective tool for the forensic identification of human specimens without requiring sophisticated laboratory instruments.

Keywords

Loop-mediated isothermal amplification (LAMP) Human DNA test Gold nanoparticle probe LAMP-AuNP 

Notes

Acknowledgments

Khanistha Watthanapanpituck was supported by the Australian Federal Police (AFP) Forensic Science Scholarship 2011. This project was approved by Mahidol University Institutional Review Board (MU-IRB) by an Expedited Review Process (COA. No. MU-IRB 2012 137.0708).

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Khanistha Watthanapanpituck
    • 1
    • 5
  • Wansika Kiatpathomchai
    • 2
    • 3
  • Eric Chu
    • 1
  • Nathinee Panvisavas
    • 1
    • 4
    Email author
  1. 1.Forensic Science Program, Multidisciplinary Unit, Faculty of ScienceMahidol UniversityBangkokThailand
  2. 2.National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development AgencyPathum ThaniThailand
  3. 3.CENTEX Shrimp, Faculty of ScienceMahidol UniversityBangkokThailand
  4. 4.Department of Plant Science, Faculty of ScienceMahidol UniversityBangkokThailand
  5. 5.Central Institute of Forensic Science, Ministry of JusticeThe Government Complex Commemorating His Majesty the King’s 80th Birthday AnniversaryBangkokThailand

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