Assessment of application value of 19 autosomal short tandem repeat loci of GoldenEyeTM 20A kit in forensic paternity testing
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This study was carried out to assess the application value of 19 autosomal short tandem repeat (STR) loci of GoldenEyeTM 20A kit, in which 13 combined DNA index system core STR loci and PentaE, PentaD, D2S1338, D19S433, D12S391, and D6S1043 of six STR loci could be used in forensic paternity testing in Chinese population. We amplified the genomic DNA from blood samples on FTA paper of 289 paternity testing cases by using the GoldenEyeTM 20A kit. The amplified products were detected by capillary electrophoresis, and then the genotypes of 20 genetic markers including 19 STR loci as well as Amelogenin for sex determination were analyzed by GeneMapper v3.2 and GeneMarker HID Software. The results of genotypes were compared to the three commonly used commercial kits including AmpFℓSTR IdentifilerTM, PowerPlexTM16, and AmpFℓSTR SinofilerTM kits. Compared to the three other common commercial kits, the GoldenEyeTM 20A kit had higher value of combined paternity index in certainty of paternity or non-exclusion paternity cases, and more numbers of STR loci were excluded in exclusionary paternity cases. Our data in this study showed that the GoldenEyeTM 20A kit has a higher application value in forensic paternity testing and will be of help for kinship analysis.
KeywordsForensic genetics GoldenEyeTM 20A kit Short tandem repeat (STR) Paternity testing Assessment
This study was supported by a grant from the Foundation for the Peoples with Highly Education, Xinxiang Medical University (no. 2005). We would like to express our acknowledgment to adjunct associate professor Wancai Yang, Department of Pathology, University of Illinois at Chicago, for improving the English presentation.
Conflict of interest
The authors declare that they have no conflict of interest.
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