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International Journal of Legal Medicine

, Volume 127, Issue 3, pp 593–601 | Cite as

Determination of psilocin, bufotenine, LSD and its metabolites in serum, plasma and urine by SPE-LC-MS/MS

  • Rafaela MartinEmail author
  • Jennifer Schürenkamp
  • Angela Gasse
  • Heidi Pfeiffer
  • Helga Köhler
Original Article

Abstract

A validated method for the simultaneous determination of psilocin, bufotenine, lysergic acid diethylamide and its metabolites in serum, plasma and urine using liquid chromatography–electrospray ionization/tandem mass spectrometry was developed. During the solid-phase extraction procedure with polymeric mixed-mode cation exchange columns, the unstable analytes were protected by ascorbic acid, drying with nitrogen and exclusion of light. The limits of detection and quantitation for all analytes were low. Recovery was ≥86 % for all analytes and no significant matrix effects were observed. Interday and intraday imprecisions at different concentrations ranged from 1.1 to 8.2 % relative standard deviation, bias was within ±5.3 %. Processed samples were stable in the autosampler for at least 2 days. Furthermore, freeze/thaw and long-term stability were investigated. The method was successfully applied to authentic serum and urine samples.

Keywords

Hallucinogens LC-MS/MS Solid-phase extraction Validation 

Notes

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Rafaela Martin
    • 1
    Email author
  • Jennifer Schürenkamp
    • 1
  • Angela Gasse
    • 1
  • Heidi Pfeiffer
    • 1
  • Helga Köhler
    • 1
  1. 1.Institute of Legal MedicineUniversity Hospital MünsterMünsterGermany

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