Determination of time since death is one of the most difficult and crucial issue in forensic medicine. Apart from body cooling, which is commonly used in the early postmortem interval (PMI), supravital reactions are the most interesting postmortem changes for time of death estimation. Nasal ciliary motility has been occasionally observed in postmortem period although no studies have focused on this phenomenon for forensic purposes. We aimed to evaluate the diagnostic usefulness of ciliary motility as a potential tool in estimating the time of death. Specimens of ciliated epithelium from 100 consecutive cadavers were obtained by scraping the nasal mucosa at three different postmortem intervals. The samples were then smeared on a slide, and an in vitro evaluation of ciliary movement was analyzed by phase-contrast microscopy. A postmortem nasal ciliary motility was observed, and a statistically significant relationship between decreasing ciliary movements and increasing postmortem interval was detected even in presence of putrefactive changes of nasal ultrastructure integrity. Some peculiar causes of death seem to influence ciliary motility in the early PMI, while no significant correlations with sex or age were observed. According to the results of this study, postmortem evaluation of nasal ciliary motility may be a bona fide and a feasible option for estimating the time of death.
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Lundquist F (1956) Physical and chemical methods for the estimation of the time of death. Acta Med Leg Sog Liege 9:205–213
Madea B (2005) Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry? Forensic Sci Int 151:139–149
Marshall TK, Hoare FE (1962) Estimating the time of death—the rectal cooling after death and its mathematical expression. J Forensic Sci 7(1):56–81
Henßge C (1981) Todeszeitschätzungen durch die mathematische Beschreibung der rektalen Leichenabkühlung unter verschiedenen Abkühlbedingungen. Z Rechtsmed 87:147–178
Henßge C, Brinkmann B, Püschel K (1984) Todeszeitbestimmung durch Rektaltemperaturmessungen bei Wassersuspension der Leiche. Z Rechtsmed 92:255–276
Henßge C, Althaus L, Bolt J, Freislederer A, Haffner HT, Henßge CA, Hoppe B, Schneider V (2000) Experiences with a compound method for estimating the time since death. I. Rectal temperature nomogram for time since death. Int J Leg Med 113(6):303–319
Henßge C, Althaus L, Bolt J, Freislederer A, Haffner HT, Henßge CA, Hoppe B, Schneider V (2000) Experiences with a compound method for estimating the time since death. II. Integration of non-temperature-based methods. Int J Leg Med 113(6):320–331
Henßge C, Madea B (2004) Estimation of the time since death in the early postmortem period. Forensic Sci Int 144:167–175
Henßge C, Knight B, Krompecher T, Madea B, Nokes L (2002) The estimation of the time since death in the early postmortem period. Edward Arnold, London
Muggenthaler H, Sinicina I, Hubig M, Mall G (2011) Database of post-mortem rectal cooling cases under strictly controlled conditions: a useful tool in death time estimation. Int J Leg Med 126(1):79–87
Hubig M, Muggenthaler H, Mall G (2011) Influence of measurement errors on temperature-based death time determination. Int J Leg Med 125(4):503–517
Henßge C (1988) Death time estimation in case work. I. The rectal temperature time of death nomogram. Forensic Sci Int 38:209–236
Henßge C (1992) Rectal temperature time of death nomogram: dependence of corrective factors on the body weight under stronger thermic insulation conditions. Forensic Sci Int 54:51–56
Zhou C, Byard RW (2011) Factors and processes causing accelerated decomposition in human cadavers—an overview. J For Leg Med 18:6–9
Madea B (1994) Importance of supravitality in forensic medicine. Forensic Sci Int 69:221–241
Gelardi M (2007) Atlas of nasal cytology for the differential diagnosis of nasal diseases. Centro Scientifico Editore, Torino 21–26 e 16–17
Caruso G, Gelardi M, Passali G, De Santi M (2007) Nasal scraping in diagnosing ciliary dyskinesia. Am J Rhinol 21(6):702–705
Lee R, Rossman C, O’brodovich H (1987) Assessment of post-mortem respiratory ciliary motility and ultrastructure. Am Rev Respir Dis 136:445–447
Hilding AC (1957) Ciliary streaming in the lower respiratory tract. Am J Physiol 191:404–410
Mayer M, Neufeld B (1980) Post-mortem changes in skeletal muscle protease and creatine phosphokinase activity—a possible marker for determination of time of death. Forensic Sci Int 15(3):197–203
Madea B, Henßge C (1990) Electrical excitability of skeletal muscle postmortem in case work. Forensic Sci Int 47(3):207–227
Püschel K, Brinkmann B, Lieske K (1985) Ultrastructural alterations of skeletal muscles after electric shock. Am J Forensic Med Pathol 6(4):296–300
Madea B (2002) Post-mortem mechanical excitation of skeletal muscle. In: Henßge C, Knight B, Krompecher T, Madea B, Nokes L (eds) The estimation of the time since death in the early post-mortem period. Edward Arnold, London, pp 160–164
Madea B (2002) Post-mortem electrical excitability of skeletal muscle in case-work. In: Henßge C, Knight B, Krompecher T, Madea B, Nokes L (eds) The estimation of the time since death in the early post-mortem period. Edward Arnold, London, pp 164–206
Madea B, Oehmichen M, Henßge C (1986) Postmortem transport of gastric contents? Z Rechtsmed 97(3):201–206
Jaffe FA (1989) Stomach contents and the time of death. Reexamination of a persistent question. Am J Forensic Med Pathol 10(1):37–41
Collins KA, Bennett AT (2001) Persistence of spermatozoa and prostatic acid phosphatase in specimens from deceased individuals during varied post-mortem intervals. Am J Forensic Med Pathol 22(3):228–232
Baccino E, De Saint ML, Schuliar Y, Guilloteau P, Le Rhun M, Morin JF, Leglise D, Amice J (1996) Outer ear temperature and time of death. Forensic Sci Int 83:133–146
Cattaneo C, Di Giancamillo A, Campari O, Martrille L, Jouineau C (2009) Infrared tympanic thermography as a substitute for a probe in the evaluation of ear temperature for postmortem interval determination: a pilot study. J Forensic Leg Med 16:215–217
Jashnani KD, Kale SA, Rupani AB (2010) Vitreous humor: biochemical constituents in estimation of postmortem interval. J Forensic Sci 55(6):1523–1527
Estracanholli ES, Kurachi C, Vicente JR, Campos De Menezes PF, Castro O, Junior S, Bagnato VS (2009) Determination of postmortem interval using in situ optical fluorescence. Opt Express 17(10):8185–8192
Singh D, Prashad R, Sharma S, Pandey AN (2006) Estimation of post-mortem interval from human pericardial fluid electrolytes concentrations in Chandigarh zone of India: log transformed linear regression model. Leg Med 8:279–287
Yadav J, Deshpande A, Arora A, Athawal BK, Dubey BP (2007) Estimation of time since death from CSF electrolyte concentration in Bhopal region of central India. Leg Med 9:309–313
Bush A, Chodhari R, Collins N, Copeland F, Hall P, Harcourt J, Hariri M, Hogg C, Lucas J, Mitchison HM, O’Callaghan C, Phillips G (2007) Primary ciliary dyskinesia: current state of the art. Arch Dis Child 92(12):1136–1140
Berbari NF, O’Connor AK, Haycraft CJ, Yoder BK (2009) The primary cilium as a complex signaling center. Curr Biol 19(13):R526–R535
Thomas B, Rutman A, O’Callaghan C (2009) Disrupted ciliated epithelium shows ciliary beat frequency and increased dyskinesia. Eur Respirt J 34:401–404
Clary-Meinesz CF, Cosson J, Huitorel P, Blaive B (1992) Temperature effect on the ciliary beat frequency of human nasal and tracheal ciliated cells. Biol Cell 76(3):335–338
Armengot M, Milara J, Mata M, Carda C, Cortijo J (2010) Cilia motility and structure in primary and secondary ciliary dyskinesia. Am J Rhinol Allergy 24(3):175–180
We wish to thank Dr. Antonio Moschetta for his critical review of the manuscript.
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Romanelli, M.C., Gelardi, M., Fiorella, M.L. et al. Nasal ciliary motility: a new tool in estimating the time of death. Int J Legal Med 126, 427–433 (2012). https://doi.org/10.1007/s00414-012-0682-x
- Nasal ciliary motility
- Forensic medicine
- Postmortem interval
- Time of death