Abstract
The unique case of a 50-year-old known alcoholic whose corpse was exhumed 27 years after death is reported. The man apparently committed suicide by hanging, but many years later the case was questioned and homicide—linked to a long-lasting serial killer case—was suspected. Thus, the corpse was exhumed, and at the autopsy it was found to be naturally mummified. This fact permitted the analysis of body tissues with the aim to investigate the persistence of ethanol conjugates in the biological material 27 years after death. Fragments of liver and kidney, a blood clot, and a hair strand were collected and submitted to liquid chromatography tandem mass spectrometry analysis. Ethyl glucuronide (EtG) and ethyl sulfate (EtS) were identified and quantified in the liver, the kidney, and the blood clot. Hair analysis was found to be severely affected by ion suppression even after solid phase extraction. Consequently, EtG was identified in all hair segments (0–3 cm, 3–6 cm, and 6–10 cm), but no reliable quantification could be carried out. In summary, our findings demonstrate that, notwithstanding the expected conjugate degradation, EtG and EtS can be indicative of ante-mortem use of alcohol even many years after death.
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Høiseth G, Karinen R, Christophersen AS, Olsen L, Normann PT, Mørland J (2007) A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol. Forensic Sci Int 165:41–45
Schloegl H, Dresen S, Spaczynski K, Stoertzel M, Wurst FM, Weinmann W (2006) Stability of ethyl glucuronide in urine, post-mortem tissue and blood samples. Int J Legal Med 120:83–88
Høiseth G, Karinen R, Johnsen L, Normann PT, Christophersen AS, Mørland J (2008) Disappearance of ethyl glucuronide during heavy putrefaction. Forensic Sci Int 176:147–151
Helander A, Dahl H (2005) Urinary tract infection: a risk factor for false-negative urinary ethyl glucuronide but not ethyl sulfate in the detection of recent alcohol consumption. Clin Chem 51:1728–1730
Helander A, Olsson I, Dahl H (2007) Postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumption. Clin Chem 53:1855–1857
Morini L, Politi L, Zucchella A, Polettini A (2007) Ethyl glucuronide and ethyl sulphate determination in serum by liquid chromatography-electrospray tandem mass spectrometry. Clin Chim Acta 376:213–219
Morini L, Politi L, Groppi A, Stramesi C, Polettini A (2006) Determination of ethyl glucuronide in hair samples by liquid chromatography/electrospray tandem mass spectrometry. J Mass Spectrom 41:34–42
Schloegl H, Rost T, Schmidt W, Wurst FM, Weinmann W (2006) Distribution of ethyl glucuronide in rib bone marrow, other tissues and body liquids as proof of alcohol consumption before death. Forensic Sci Int 156:213–218
Halter CC, Dresen S, Auwaerter V, Wurst FM, Weinmann W (2008) Kinetics in serum and urinary excretion of ethyl sulfate and ethyl glucuronide after medium dose ethanol intake. Int J Legal Med 122:123–128
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Politi, L., Morini, L., Mari, F. et al. Ethyl glucuronide and ethyl sulfate in autopsy samples 27 years after death. Int J Legal Med 122, 507–509 (2008). https://doi.org/10.1007/s00414-008-0273-z
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DOI: https://doi.org/10.1007/s00414-008-0273-z