Abstract
When a body is exposed to a cold environment, the livid colour of livor mortis changes to cherry red. This colour change is due to an increase in the concentration of oxygenated haemoglobin. The chronological course and the extent of haemoglobin re-oxygenation associated with the exposure to low ambient temperatures have not been understood so far. The relations between refrigeration time under a constant ambient temperature (5°C), skin temperature, body mass index (BMI), spectral reflectance curve and O2–Hb concentration in livor mortis were systematically investigated in 84 bodies brought to the Institute of Legal Medicine of the Freiburg University Hospital shortly after death. In the first measurements performed shortly after death, the reflectance curves of the livores of all bodies showed a broad minimum at 555 nm. After a refrigeration time of 44.9 ± 17.9 h, the spectrum changed to the typical picture of O2-rich blood with 2 minima at 541 and 576 nm and a maximum at 560 nm in between. This qualitative change of the reflectance spectra was observed for a skin temperature of 10.3 ± 2.7°C. With the help of a physical skin model it was possible to calculate that due to the post-mortem exposure to cold the O2–Hb concentration in the livores rose from 0–1% to a value of up to 89.3%. The change in the reflectance curve was discernible from an oxygen saturation of 25 ± 13.8%.
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References
Belenkaia L, Bohnert M, Liehr AW (2006) Electronic laboratory notebook assisting reflectance spectrometry in legal medicine. arXiv DOI http://www.arxiv.org/abs/cs.DB/0612123
Bohnert M, Weinmann W, Pollak S (1999) Spectrophotometric evaluation of postmortem lividity. Forensic Sci Int 99:149–158
Bohnert M, Walther R, Roths T, Honerkamp J (2005) A Monte Carlo-based model for steady state diffuse reflectance spectrometry in human skin: estimation of carbon monoxide concentration in livor mortis. Int J Legal Med 119:355–362
Bolin FP, Preuss LE, Taylor CR, Ference RJ (1989) Refractive index of some mammalian tissues using a fibre optic cladding method. Appl Opt 28:2297–2303
Brinkmann B, Madea B (eds) (2004) Handbuch Gerichtliche Medizin. Springer, Berlin
Di Maio VJM, Di Maio DJ (2001) Forensic pathology, 2nd edn. CRC, Boca Raton, FL
Forster B (1987) Praxis der Rechtsmedizin. Thieme, Stuttgart New York
Guyton AC, Hall JE (2005) Textbook of medical physiology, 11th edn. Saunders, London
Henssge C, Knight B, Krompecher T, Madea B, Nokes L (1995) The estimation of the time since death in the early postmortem period. Arnold, London
Henssge C, Althaus L, Bolt J et al (2000) Experiences with a compound method for estimating the time since death. I. Rectal temperature nomogram for time since death. Int J Legal Med 113:303–309
Henssge C, Madea B (2004) Estimation of the time since death in the early post-mortem period. Forensic Sci Int 144:167–175
Holzer FJ (1934) Über Eigentümlichkeiten beim Rotwerden der Totenflecken. Z Medizinalbeamt 2:65–72
Kaatsch HJ, Stadler M, Nietert M (1993) Photometric measurement of color changes in livor mortis as a function of pressure and time. Development of a computer-aided system for measuring pressure-induced blanching of livor mortis to estimate time of death. Int J Legal Med 106:91–97
Kaatsch HJ, Schmidtke W, Nietsch W (1994) Photometric measurement of pressure-induced blanching of livor mortis as an aid to estimating time of death. Application of a new system for quantifying pressure-induced blanching in lividity. Int J Legal Med 106:209–214
Kessler H (1951) Zur Differentialdiagnose der Einwirkung von Kälte oder Kohlenoxyd auf die Färbung der Hypostase. M.D. thesis, University of Heidelberg
Lins G (1973) Der Farbort der Totenflecken im Spektralfarbenzug. Beitr Gerichtl Med 31:203–212
Maeda H, Fukita K, Oritani S, Ishida K, Zhu BL (1997) Evaluation of post-mortem oxymetry with reference to the causes of death. Forensic Sci Int 87:201–210
Mall G, Hubig M, Beier G, Eisenmenger W (1998) Energy loss due to radiation in postmortem cooling. Part A: quantitative estimation of radiation using the Stefan–Boltzmann law. Int J Legal Med 111:299–304
Mall G, Hubig M, Beier G, Büttner A, Eisenmenger W (1999) Energy loss due to radiation in postmortem cooling. Part B: energy balance with respect to radiation. Int J Legal Med 112:233–240
Mall G, Eckl M, Sinicina I, Peschel O, Hubig M (2004) Temperature-based death time estimation with only partially known environmental conditions. Int J Legal Med 119:185–194
Mueller B (1975) Gerichtliche Medizin, 2nd edn. Springer, Heidelberg
Perelman LT, Backman V, Wallace M et al (1998) Observation of periodic fine structure in reflectance from biological tissue: a new technique for measuring nuclear size distribution. Phys Rev Lett 80:627–630
Prokop O, Göhler W (1976) Forensische Medizin, 3rd edn. Fischer, Stuttgart New York
Saukko PJ, Knight B (eds) (2004) Knight’s forensic pathology, 3rd edn. Arnold, London
Schuller E, Pankratz H, Liebhardt E (1987) Farbortmessungen an Totenflecken. Beitr Gerichtl Med 45:169–173
Schuller E, Pankratz H, Wohlrab S, Liebhardt E (1988) Die Bestimmung des Farbortes der Totenflecken in Beziehung zur Wegdrückbarkeit. In: Bauer G (ed) Gerichtsmedizin. Festschrift für Wilhelm Holczabeck. Deuticke, Vienna, pp 295–302
Schwerd W (1992) Rechtsmedizin, 5th edn. Deutscher Ärzte-Verlag, Köln
Spitz WU (ed) (1993) Medicolegal investigation of death, 3rd edn. Thomas, Springfield, IL
Vanezis P (1991) Assessing hypostasis by colorimetry. Forensic Sci Int 52:1–3
Vanezis P, Trujillo O (1996) Evaluation of hypostasis using a colorimeter measuring system and its application to assessment of the post-mortem interval (time of death). Forensic Sci Int 78:19–28
Walther R, Roths T, Bohnert M, Honerkamp J (2004) Monte Carlo based model for steady-state diffuse reflectance spectroscopy. Proc SPIE 5261:70–81
Zonios G, Perelman LT, Backman V, Manoharan R, Fitzmaurice M, Dam JV, Feld MS (1999) Diffuse reflectance spectroscopy of human adenomatous colon polyps in vivo. Appl Opt 38:6628–6637
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This study was supported by the Deutsche Forschungsgemeinschaft (German Research Council), file number BO 1923/2-1.
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Bohnert, M., Schulz, K., Belenkaia, L. et al. Re-oxygenation of haemoglobin in livores after post-mortem exposure to a cold environment. Int J Legal Med 122, 91–96 (2008). https://doi.org/10.1007/s00414-007-0162-x
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DOI: https://doi.org/10.1007/s00414-007-0162-x