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Population study and evaluation of 20 Y-chromosome STR loci in Germans

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Abstract

The nine European minimal haplotype (EMH) loci, the two SWGDAM loci and five further single-copy Y-chromosomal short tandem repeats (Y-STRs) DYS446, DYS447, DYS448, DYS449, DYS463, and the multicopy loci DYS464 were evaluated in the German population groups Dresden, Hamburg, Rostock, Munich, and the Sorbs who are a Slavic-speaking minority in Lusatia. Highest gene diversities in all populations were shown for DYS464, DYS385, and DYS449 (D=0.8559–0.9486). The haplotype diversity for the European minimal haplotype loci ranged between 0.9852 for Sorbs and 0.9983 for the Hamburg population showing that there is a significant portion of haplotypes which could not be resolved. Advanced typing using DYS446, DYS447, DYS448, DYS449, DYS463, and DYS464 discriminated all non-related individuals of the Dresden, Hamburg, and Rostock populations. Evaluation of the Y-STRs was accomplished by sequence analysis of all allelic fragments of the allelic ladders and microvariant alleles of DYS385 and the determination of the amounts of stutter products of the loci.

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Acknowledgements

This work was supported by the German Federal Ministry of Education and Research (BMBF, project number 03i4008). The authors would like to thank Lutz Roewer for kind cooperation. We also thank all the DNA donors and Christa Augustin, Volker Weirich, Burkhard Rolf, and Ivana Furac for kindly provided DNA samples. We acknowledge Stefanie Weidlich, Susann Gerber, and Mareen Müller for excellent technical assistance.

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Authors and Affiliations

  1. Biotype AG, Moritzburger Weg 67, 01109, Dresden, Germany

    Heike Rodig, Manja Grum & Hans-Dieter Grimmecke

Authors
  1. Heike Rodig
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  2. Manja Grum
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  3. Hans-Dieter Grimmecke
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Corresponding author

Correspondence to Heike Rodig.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1

Allele frequencies and gene diversities for Y-STR loci in Germans (PDF 22 kb)

Supplementary Table 2

Y-chromosome haplotype distribution in 235 individuals from 5 German population groups (PDF 31 kb)

Supplementary Table 3

Ranking list of Y-STR loci with regard to high values of gene diversity (PDF 8 kb)

Supplementary Table 4

Haplotype diversities of 5 German populations. European Minimal Haplotype loci (EMH), SWGDAM loci, and loci of the RAR multiplex were analyzed (PDF 7 kb)

Supplementary Table 5

Stutter artefacts of Y-STRs . The peak areas were used to calculate the relative amount of the stutter artefacts (% stutter) in comparison to the allele specific product (PDF 5 kb)

Supplementary Table 6

Sequencing data of allelic ladders and haplotypes of reference DNAs (PDF 10 kb)

Supplementary Table 7

Microvariant alleles of DYS385 (PDF 5 kb)

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Rodig, H., Grum, M. & Grimmecke, HD. Population study and evaluation of 20 Y-chromosome STR loci in Germans. Int J Legal Med 121, 24–27 (2007). https://doi.org/10.1007/s00414-005-0075-5

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  • DOI: https://doi.org/10.1007/s00414-005-0075-5

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