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DXS10079, DXS10074 and DXS10075 are STRs located within a 280-kb region of Xq12 and provide stable haplotypes useful for complex kinship cases

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The evaluation of the short tandem repeat (STR) markers DXS10079, DXS10074 and DXS10075 was amended to establish a STR cluster spanning a genetic distance<1 cM. These three STRs are located within a 280-kb region at Xq12 and provide stable haplotypes useful for solving complex kinship cases. Theoretically, this cluster could give rise to 2,548 different haplotypes in the German population and the genotyping of 781 men revealed the presence of 172 haplotypes. Since the three STRs were shown to be in strong linkage disequilibrium (LD), haplotype frequencies cannot be computed on the basis of a single locus allele frequency alone but have to be estimated directly. Here, we present data on linkage, haplotype frequencies and LD in a German population. Further clusters from other regions of the X chromosome will be published in the future to cover the chromosome with a well-structured network of highly informative sites.

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  1. Bär W, Brinkmann B, Budowle B et al (1997) DNA recommendations—further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. Int J Legal Med 110:175–176

    Article  PubMed  Google Scholar 

  2. Blyth C, Still H (1983) Binomial confidence intervals. J Am Stat Asso 78:108–116

    Article  Google Scholar 

  3. Brinkmann B, Klintschar M, Neuhuber F, Huhne J, Rolf B (1998) Mutation rate in human microsatellites: influence of the structure and length of the tandem repeat. Am J Hum Genet 62:1408–1415

    Article  PubMed  CAS  Google Scholar 

  4. Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templated nucleotide addition by Taq DNA polymerase: primer modifications that facilitate genotyping. Biotechniques 20:1004–1006, 1008–1010

    PubMed  CAS  Google Scholar 

  5. Casella G (1986) Refining binomial confidence intervals. Can J Stat 14:113–129

    Google Scholar 

  6. Clayton TM, Guest JL, Urquhart AJ, Gill PD (2004) A genetic basis for anomalous band patterns encountered during DNA STR profiling. J Forensic Sci 49:1207–1214

    Article  PubMed  CAS  Google Scholar 

  7. Crouse CA, Rogers S, Amiott E, Gibson S, Masibay A (1999) Analysis and interpretation of short tandem repeat microvariants and three-banded allele patterns using multiple allele detection systems. J Forensic Sci 44:87–94

    PubMed  CAS  Google Scholar 

  8. Edelmann J, Hering S, Michael M et al (2001) 16 X-chromosome STR loci frequency data from a German population. Forensic Sci Int 124:215–218

    Article  PubMed  Google Scholar 

  9. Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222

    Article  PubMed  CAS  Google Scholar 

  10. Edelmann J, Deichsel D, Plate I, Kaser M, Szibor R (2003) Validation of the X-chromosomal STR DXS6809. Int J Legal Med 117:241–244

    Article  PubMed  CAS  Google Scholar 

  11. Guo SW, Thompson EA (1992) Performing the exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics 48:361–372

    Article  PubMed  CAS  Google Scholar 

  12. Hering S, Szibor R (2000) Development of the X-linked tetrameric microsatellite marker DXS9898 for forensic purposes. J Forensic Sci 45:929–931

    PubMed  CAS  Google Scholar 

  13. Hering S, Brundirs N, Kuhlisch E et al (2004) DXS10011: studies on structure, allele distribution in three populations and genetic linkage to further q-telomeric chromosome X markers. Int J Legal Med 118:313–319

    Article  PubMed  Google Scholar 

  14. Kayser M, Roewer L, Hedman M et al (2000) Characteristics and frequency of germline mutations at microsatellite loci from the human Y chromosome, as revealed by direct observation in father/son pairs. Am J Hum Genet 66:1580–1588

    Article  PubMed  CAS  Google Scholar 

  15. Nei M, Roychoudhury AK (1974) Sampling variances of heterozygosity and genetic distance. Genetics 76:379–390

    PubMed  CAS  Google Scholar 

  16. Poetsch M, Repenning A, Lignitz E, Kuhlisch E, Szibor R (2005) DXS6797 contains two STRs which can be easily haplotyped in both sexes. Int J Legal Med. DOI 10.1007/s00414-005-0003-8:1–6

  17. Szibor R, Krawczak M, Hering S, Edelmann J, Kuhlisch E, Krause D (2003) Use of X-linked markers for forensic purposes. Int J Legal Med 117:67–74

    PubMed  CAS  Google Scholar 

  18. Szibor R, Hering S, Edelmann J (2005) A new Web-site compiling forensic chromosome X research is now online. Int J Legal Med. DOI 10.1007/s00414-005-0029-y

  19. Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119(6):363–369

    Article  PubMed  Google Scholar 

  20. Talbot CC Jr, Avramopoulos D, Gerken S, Chakravarti A, Armour JA, Matsunami N, White R, Antonarakis SE (1995) The tetranucleotide repeat polymorphism D21S1245 demonstrates hypermutability in germline and somatic cells. Hum Mol Genet 4:1193–1199

    PubMed  CAS  Google Scholar 

  21. Zamir A, Shpitzen M, Oz C, Motro U, Meiner V, Gafny R (2002) Presentation of a three-banded allele pattern-analysis and interpretation. J Forensic Sci 47:824–826

    PubMed  Google Scholar 

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Correspondence to Reinhard Szibor.

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Hering, S., Augustin, C., Edelmann, J. et al. DXS10079, DXS10074 and DXS10075 are STRs located within a 280-kb region of Xq12 and provide stable haplotypes useful for complex kinship cases. Int J Legal Med 120, 337–345 (2006).

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