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Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay

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Abstract

One hundred fifty-five unrelated, autochthonous healthy males from Northeast Italy were typed for the 17 Y-chromosome short tandem repeat (STR) (Y-STR) loci DYS456, DYS389I, DYS390, DYS389II, DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635, DYS392, Y GATA H4, DYS437, DYS438, DYS448 using the AmpFLSTR Yfiler polymerase chain reaction amplification kit. A total of 153 different haplotypes were observed, and among these, 151 were unique, while 2 were found two times. The overall haplotype diversity was 0.9997. Furthermore, 50 father–son pairs, previously confirmed by autosomal STR analysis, were typed using the same set of 17 Y-STR loci, and, among 850 allele transfers, three mutation events were identified, giving an average mutation rate of 3.53×10−3 per locus per generation (95% confidence interval 0.73–1.03).

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Acknowledgements

This work was supported by a grant from M.I.U.R. and “Donazione Miria Cherubini e Amleto Loro.”

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Authors and Affiliations

  1. Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi, Piazzale L.A. Scuro, 37134, Verona, Italy

    Stefania Turrina, Renzo Atzei & Domenico De Leo

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  1. Stefania Turrina
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  2. Renzo Atzei
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  3. Domenico De Leo
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Correspondence to Domenico De Leo.

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Turrina, S., Atzei, R. & De Leo, D. Y-chromosomal STR haplotypes in a Northeast Italian population sample using 17plex loci PCR assay. Int J Legal Med 120, 56–59 (2006). https://doi.org/10.1007/s00414-005-0054-x

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  • DOI: https://doi.org/10.1007/s00414-005-0054-x

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