Abstract
In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-μl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-μl reaction volumes.
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Acknowledgements
We greatly acknowledge the excellent laboratory assistance of Tanja Schmitt (Alopex GmbH), who carried out the on-chip amplification reactions.
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U. Schmidt and S. Lutz-Bonengel contributed equally to this work.
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Schmidt, U., Lutz-Bonengel, S., Weisser, HJ. et al. Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit. Int J Legal Med 120, 42–48 (2006). https://doi.org/10.1007/s00414-005-0041-2
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DOI: https://doi.org/10.1007/s00414-005-0041-2