Abstract
D-dimer, a specific fragment resulting from degradation of cross-linked fibrin, is an essential marker for the diagnosis of disseminated intravascular coagulation (DIC). Rapid assay for D-dimer using monoclonal antibody coated-latex particles might be useful for discriminating between postmortem and antemortem blood in bloodstains. We tried to detect D-dimer in nine postmortem blood samples by the rapid latex agglutination assay and to quantify them automatically using the latex photometric immunoassay system. The results showed that all samples were positive and that their amounts of D-dimer were 335–2,800 μg/ml (the normal blood level, <1 μg/ml; the pathogenic blood level with DIC, 1–100 μg/ml). Next, nine stains made of postmortem blood were examined by the rapid latex agglutination assay. The result showed that only one case (D-dimer 335 μg/ml blood) showed weak positive while the others (D-dimer 600–2,800 μg/ml blood) were positive. The present study indicates that the latex agglutination assay for D-dimer can be useful to demonstrate the presence of postmortem blood.
References
Akman MN, Cetin N, Bayramoglu M, Isiklar I, Kilinic S (2004) Value of the D-dimer test in diagnosing deep vein thrombosis in rehabilitation inpatients. Arch Phys Med Rehabil 85(7):1091–1094
Arai M (1986) Japanese title [D-dimer ni taisuru monokuronarukoutai wo mochiita FDP no bunnkakuteiryouhou no kentou]. Rinsho Byori 34:157–161
Baroldi G, Mittleman RE, Parolini M, Silver MD, Fineschi V (2001) Myocardial contraction bands. Definition, quantification and significance in forensic pathology. Int J Leg Med 115:142–151
Bates SM, Grand’Maison A, Johuston M, Naguit I, Kavacs MJ, Ginsberg JS (2001) A latex D-dimer reliably excludes venous thromboembolism. Arch Intern Med 161(3):447–453
Bonelli A, Bacci S, Vannelli GB, Norelli GA (2003) Immunohistochemical localization of mast cells as a tool for the discrimination of vital and postmortem lesions. Int J Leg Med 117:14–18
Elms MJ (1983) Measurement of crosslinked fibrin degradation products—an immunoassay using monoclonal antibodies. Thromb Haemost 50(2):591–594
Elms MJ, Bunce IH, Bundesen PG, Rylatt DB, Webber AJ, Masci PP, Whitaker AN (1986) Rapid detection of cross-linked fibrin degradation products in plasma using monoclonal antibody-coated latex particles. J Clin Pathol 85:360–364
Francis CW, Marder VJ, Martin SE (1980) Plasmic degradation of crosslinked fibrin. I. Structural of the particulate clot and identification of new macromolecular soluble complexes. Blood 56:456–464
Hernandez-Cueto C, Girela E, Sweet DJ (2000) Advances in the diagnosis of wound vitality: a review. Am J Forensic Med Pathol 21:21–31
Hunt FA, Rylatt DB, Hart RA, Bundesen PG (1985) Serum crosslinked fibrin (XDP) and fibrinogen/fibrin degradation products (FDP) in disorders associated with activation of the coagulation or fibrinolytic systems. Br J Haematol 60:715–722
Ikematsu S, Kuroso K (1989) Comparative evaluation of D-dimer assays. Rinsho Byori 81:189–197
Kuroso K (1986) Japanese title [anteika fiburin bunkaisanbutsu DD/E complex no sokuteihou no kaihatsu to senyoukatei ni okeru kougensei no henka ni tsuite]. Tokyo Med Univ Zasshi 44:860–871
Mihalyi E (1983) Kinetics and molecular mechanism of the proteolytic fragmentation of fibrinogen. Ann NY Acad Sci 408:60–69
Miyashita S (1994) Discrimination between postmortem and antemortem blood by dot-ELISA for human myoglobin. Jpn J Leg Med 48(6):433–438
Muraoka S (1980) Studies on the blood stains by scanning electron microscopy. Jpn J Leg Med 34(6):605–617
Ortiz-Rey JA, Suárez-Peñaranda JM, Muñoz-Barús JI, Álvarez C, San Miguel P, Rodriguez-Calvo MS, Concheiro-Carro L (2003) Expression of fibronectin and tenascin as a demonstration of vital reaction in rat skin and muscle. Int J Leg Med 117:356–360
Ortmann C, Pfeiffer H, Brinkmann B (2000) Demonstration of myocardial necrosis in the presence of advanced putrefaction. Int J Leg Med 114:50–55
Rutty GN, Woolley A, Brookfield C, Shepherd F, Kitchen S (2003) The PIVKA II test. The first reliable coagulation test for autopsy investigations. Int J Leg Med 117:143–148
Sanaka A (1953) On the process that the blood of the cadavers of those having suddenly died acquires fluidity. Jpn J Leg Med 7(1):27–32
Satoh K, Hayakawa S, Nakanishi K (1975) Identification of antemortem and postmortem blood. Jpn J Leg Med 29(4):209–283
Shinbo K, Yokozuka H, Yakahashi K, Komiya M, Oikawa S, Ieiri T (1997) A fundamental evaluation of blood coagulation and fibrinolysis markers assay using a fully automated immunoassay system, LPIA-200. Igaku to Yakugaku 37(1):157–162
Sugie H, Nihikawa T, Funao T (1995) Quantification of nucleotides, nucleosides and bases in antemortem and postmortem bloodstains by high-performance liquid chromatography. Forensic Sci Int 71:123–130
Wada H, Sase T, Matsumoto T, Kushiya F, Sakakura M, Mori Y, Nishikawa M, Ohnishi K, Nakatani K (2003) Increasing soluble fibrin in plasma of patients with disseminated intravascular coagulation. Clin Appl Thromb Hemost 9(3):233–240
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Sakurada, K., Sakai, I., Sekiguchi, K. et al. Usefulness of a latex agglutination assay for FDP D-dimer to demonstrate the presence of postmortem blood. Int J Legal Med 119, 167–171 (2005). https://doi.org/10.1007/s00414-004-0517-5
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DOI: https://doi.org/10.1007/s00414-004-0517-5