Abstract
A set of seven STR-loci mapping on the male-specific region of the human Y chromosome (DYS19, DYS385, DYS389 I/II, DYS390, DYS391, DYS392, DYS393) were typed by means of two multiplex PCR reactions and capillary electrophoresis in a Romanian population sample of 104 unrelated males. Among the 97 different haplotypes observed, 92 were unique, while 5 occurred more than once. The observed haplotype diversity was 0.989.
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Beleza S, Alves C, Gonzalez-Neira A, Lareu M, Amorim A, Carracedo A, Gusmao L (2003) Extending STR markers in Y chromosome haplotypes. Int J Legal Med 117:27–33
Uchihi R, Yamamoto T, Usuda K et al. (2003) Haplotype analysis with 14 Y-STR loci using 2 multiplex amplification and typing systems in 2 regional populations in Japan. Int J Legal Med 117:34–38
Kayser M, Caglia A, Corach D et al. (1997) Evaluation of Y chromosomal STRs: a multicenter study. Int J Legal Med 110:125–133
Knijff P de, Kayser M, Caglia A et al. (1997) Chromosome Y microsatellites: population genetic and evolutionary aspects. Int J Legal Med 110:134–140
Jobling MA, Pandya A, Tyler-Smith C (1997) The Y chromosome in forensic analysis and paternity testing. Int J Legal Med 110:118–124
Stefan M, Stefanescu G, Gavrila L, Terrentano L, Jobling M, Malaspina P, Novelletto A (2001) Y chromosome analysis reveals a sharp genetic boundary in the Carpathian region. Eur J Hum Genet 9:27–33
Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10:506–513
Gill P, Brenner C, Brinkmann B et al. (2001) DNA Commission of the International Society of Forensic Genetics: recommendations on the forensic analysis using Y-chromosome STRs. Int J Legal Med 114:305–309
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Acknowledgements
The authors wish to thank all blood donors who made this study possible. The samples were obtained with the help of the medical team from the "Victor Babes" Hospital in Bucharest. Ms. Cornelia Iosef who coordinated the sampling procedures is gratefully acknowledged.
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Barbarii, L.E., Rolf, B. & Dermengiu, D. Y-chromosomal STR haplotypes in a Romanian population sample. Int J Legal Med 117, 312–315 (2003). https://doi.org/10.1007/s00414-003-0397-0
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DOI: https://doi.org/10.1007/s00414-003-0397-0