Abstract
The histamine content in vital wounds is known to increase, with a zenith after 3 h, and then decrease until 24 h after wounding. We addressed whether this biochemical alteration has a morphological counterpart. Since the main source of skin histamine are mast cells, the distribution and number of these cells was assessed upon labeling with fluorescent avidin and with antibodies to the mast cell specific enzymes, chymase and tryptase. Analyses were performed on skin from 15 healthy controls (from surgical biopsies), from 15 post-mortem lesions and 75 vital lesions, obtained at autopsy from subjects who had survived from a few seconds to 24 h. The number of mast cells per unit area of section surface increased progressively with survival time, up to a maximum in subjects who survived 1–3 h (p<0.01), and decreased thereafter becoming less than in the controls if lesions had occurred earlier than 6 h before death (p<0.01). Samples from post-mortem lesions had significantly fewer mast cells than those of any other groups of samples (p<0.01). We suggest that in association to other histological and circumstantial evidence the analysis of mast cells by affinity cytochemistry can help to discriminate vital from post-mortem lesions and to estimate survival time after lesions.
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Acknowledgements
This work was supported by a grant from the Italian Ministry of Universities and Research in Science and Technology. The authors are indebted to Prof. P. Romagnoli for discussion of the manuscript, to Prof. P. Bechi for providing part of the material under study and to Prof. G.B. Vannelli for help with fluorescence microscopy. The technical assistance of A. Forestieri, T. Venturi and P. Venturi is gratefully acknowledged.
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Bonelli, A., Bacci, S. & Norelli, G.A. Affinity cytochemistry analysis of mast cells in skin lesions: a possible tool to assess the timing of lesions after death. Int J Legal Med 117, 331–334 (2003). https://doi.org/10.1007/s00414-003-0396-1
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DOI: https://doi.org/10.1007/s00414-003-0396-1