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International Journal of Legal Medicine

, Volume 117, Issue 3, pp 170–174 | Cite as

The effectiveness of protective clothing in the reduction of potential DNA contamination of the scene of crime

  • G. N. RuttyEmail author
  • A. Hopwood
  • V. Tucker
Technical Note
  • 1.6k Downloads

Abstract

The use of ultra-sensitive low copy number (LCN) DNA typing allows the analysis of picogram amounts of DNA. Trace evidence accidentally left at a scene of crime (SOC) by the investigating team may be inadvertently collected and analysed, potentially leading to spurious evidence being introduced into the criminal investigation. A series of experiments were undertaken to determine the extent to which an investigator could contribute to any DNA contamination of a scene of crime under different simulated activities. Further, the degree to which any contamination was reduced by the use of commercially available protective clothing was demonstrated. Precautions that should routinely be taken at a scene of crime to reduce the risk of DNA contamination are recommended.

Keywords

DNA Contamination Face mask Scene suits Scene of crime 

Notes

Acknowledgements

This work was supported by the CH Milburn and Samuel Strutt British Medical Association Research and Fellowship Award.

References

  1. 1.
    Gill P, Whitaker JP, Flaxman C, Brown N, Buckleton J (2000) An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA. Forensic Sci Int 112:17–40PubMedGoogle Scholar
  2. 2.
    Oorschot RAH van, Jones MJ (1997) DNA fingerprints from fingerprints. Nature 387:767Google Scholar
  3. 3.
    Schultz MM, Reichert W (2000) A strategy for STR-analysis of cryptic epithelial cells on several textiles in practical casework. In: Sensabaugh G, Olaisen B (eds) Progress in forensic genetics 8. Elsevier Science, Amsterdam, pp 514–516Google Scholar
  4. 4.
    Rutty GN (2002) An investigation into the transference and survivability of human DNA following simulated manual strangulation with consideration of the problem of third party contamination. Int J Legal Med 116:170–173PubMedGoogle Scholar
  5. 5.
    Rutty GN, Watson S, Davison J (2000) DNA contamination of mortuary instruments and work surfaces; a significant problem in forensic practice? Int J Legal Med 114:56–60CrossRefPubMedGoogle Scholar
  6. 6.
    Rutty GN (2000) Human DNA contamination of mortuaries; does it matter? J Pathol 190:410–411CrossRefPubMedGoogle Scholar
  7. 7.
    Toledano T, Quarino L, Leung S, Buffolino P, Baum H, Shaler RC (1997) An assessment of DNA contamination risks in New York City medical examiner facilities. J Forensic Sci 42:721–724PubMedGoogle Scholar
  8. 8.
    Lowe A, Murray C, Whitaker J, Tully G, Gill P (2002) The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces. Forensic Science Int (in Press)Google Scholar
  9. 9.
    Cotton EA, Allsop R, Guest J, Frazier R, Koumi P, Callow IP, Seager A, Sparkes R (2000) Validation of the AmpFlSTR® SGMplus system for use in forensic casework. Forensic Sci Int 112:151–161PubMedGoogle Scholar
  10. 10.
    Frazier R, Millican ES, Watson SK, Oldroyd NJ, Sparkes RL, Taylor KM, Panchal S, Bark L, Kimpton CP, Gill PD (1996) Validation of the Applied Biosystems Prism 377 automated sequencer for forensic short tandem repeat analysis. Electrophoresis 17:1550–1552PubMedGoogle Scholar
  11. 11.
    Whitaker JP, Cotton EA, Gill P (2001) A comparison of the characteristics of profiles produced with the AmpFlSTR® SGMplus multiplex system for both standard and low copy number (LCN) STR DNA analysis. Forensic Science Int 123:215–223CrossRefGoogle Scholar
  12. 12.
    Schweizer RT (1976) Mask wiggling as a potential cause of wound contamination. Lancet 20:1129–1130Google Scholar
  13. 13.
    Ha'eri GB, Orth M, Wiley AM (1980) The efficacy of standard surgical face masks: an investigation using "tracer particles". Clin Orthop Relat Res 148:160–162Google Scholar
  14. 14.
    Emesley L (2000) Why wear surgical face masks? Nurs Times 96:38–39Google Scholar
  15. 15.
    McLure HA Mannam M, Talboys CA, Azadian BS, Yentis SM (2000) The effect of facial hair and sex on dispersal of bacteria below a masked subject. Anaesthesia 55:173–176CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  1. 1.Division of Forensic Pathology University of LeicesterLeicester Royal InfirmaryLeicesterUK
  2. 2.Research and DevelopmentThe Forensic Science ServiceSolihullUK

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