Abstract
Among the repetitive elements, satellite DNA (SatDNA) emerges as extensive arrays of highly similar tandemly repeated units, spanning megabases in length. Given that the satDNA PboSat01-176, previously characterized in P. boiei, prompted our interest for having a high abundance in P. boiei and potential for centromeric satellite, here, we employed various approaches, including low coverage genome sequencing, followed by computational analysis and chromosomal localization techniques in four Proceratophrys species and, investigating the genomic presence and sharing, as well as its potential for chromosomal centromere marker in Proceratophrys frog species. Our findings demonstrate that PboSat01-176 exhibits high abundance across all four Proceratophrys species, displaying distinct characteristics that establish it as the predominant repetitive DNA element in these species. The satellite DNA is prominently clustered in the peri/centromeric region of the chromosomes, particularly in the heterochromatic regions. The widespread presence of PboSat01-176 in closely related Proceratophrys species reinforces the validity of the library hypothesis for repetitive sequences. Thus, this study highlighted the utility of the satDNA family PboSat01-176 as a reliable centromeric marker in Proceratophrys species, with potential to be applied in other species of anuran amphibians. The observed sharing and maintenance of this sequence within the genus suggest possibilities for future research, particularly through expanded sampling to elucidate parameters that underlie the library hypothesis and the evolutionary dynamics of satDNA sequences.
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Acknowledgements
We would like to thank members of the Animal Cytogenetics Laboratory (UNESP) for the valuable suggestions, constructive comments, and critical reading of an earlier version of the manuscript, especially Ms. Carolina Crepaldi. We would also like to thank Prof. Diogo Cavalcanti Cabral-de-Mello for the valuable suggestions, access to genome sequencing, and access to all computational resources. We would also like to thank Prof. Célio Fernando Baptista Haddad and Dra Luiza Rieder Cholak for their kindness in collecting part of the Proceratophrys specimens analyzed in this work.
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This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP #2017/00195_7).
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MJS contributed to the work’s conception, data acquisition, analysis and interpretation, and wrote the manuscript. MJS and TG contributed to the acquisition of specimens for biological and genomic material. MJS and RFD conducted cytogenetic analysis. PPM conceptualized, funded and coordinated the research. All authors contributed to manuscript revision, read and approved the submitted version.
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The animal study was reviewed and approved by Animal Ethics Committee (Comitê de Ética no Uso de Animais - CEUA - permission 21/2019), Biosciences Institute, UNESP, Rio Claro, SP, Brazil.
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da Silva, M.J., Destro, R.F., Gazoni, T. et al. Interspecific cytogenomic comparison reveals a potential chromosomal centromeric marker in Proceratophrys frog species. Chromosoma (2024). https://doi.org/10.1007/s00412-024-00819-9
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DOI: https://doi.org/10.1007/s00412-024-00819-9