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Evolution of the parthenogenetic rock lizard hybrid karyotype: Robertsonian translocation between two maternal chromosomes in Darevskia rostombekowi

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Abstract

Darevskia rostombekowi, the most outstanding of the seven known parthenogenetic species in the genus Darevskia, is the result of an ancestral cross between two bisexual species Darevskia raddei and Darevskia portschinskii. The chromosomal set of this species includes a unique submetacentric autosomal chromosome; the origin of this chromosome was unresolved as only acrocentric chromosomes are described in the karyotypes of Darevskia genus normally. Here, we applied a suite of molecular cytogenetic techniques, including the mapping of telomeric (TTAGGG) n repeats using fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH), and whole-chromosome painting (WCP) in both D. rostombekowi and parental (D. portschinskii and D. raddei) species. The obtained results in total suggest that a de novo chromosomal rearrangement via Robertsonian translocation (centric fusion) between two maternal (D. raddei) acrocentric chromosomes of different size was involved in the formation of this unique submetacentric chromosome present in the parthenogenetic species D. rostombekowi. Our findings provide new data in specific and rapid evolutional processes of a unisexual reptile species karyotype.

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Data availability

The following information was supplied regarding data availability: All raw data are deposited online: RAW DATA Rock Lizards. https://doi.org/10.5281/zenodo.3909394.

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Acknowledgments

We would like to thank the two anonymous reviewers for their suggestions and comments. We are grateful to prof. Yu.F. Bogdanov and our colleagues I.S. Mazheĭka, A.N. Bogomazova, M.A. Lelekova, A.A. Kashintsova, and M. Asikyan for collaboration.

Funding

The reported study was funded by RFBR according to the research project № 17-00-00430 (17-00-00429), budget financing under VIGG RAS State Assignment Contract using equipment of CCU DBS RAS “Genetic polymorphism,” RFBR research project №18-54-05020, SCS MES RA-RFFR 18RF-132. MBC was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (Proc. nos 401962/2016-4 and 302449/2018-3), Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (Proc. No 2018/22033-1), and CAPES/Alexander von Humboldt (Proc. No. 88881.136128/2017-01).

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Author notes

  1. Victor Spangenberg, Oxana Kolomiets, Ilona Stepanyan, Ahmed Al-Rikabi, Thomas Liehr and Marine Arakelyan contributed equally to this work.

Authors and Affiliations

  1. Vavilov Institute of General Genetics, RAS, Gubkin 3, Moscow, 119991, Russia

    Victor Spangenberg, Oxana Kolomiets & Tatiana Grishaeva

  2. Scientific Center of Zoology and Hydroecology, Yerevan, Armenia

    Ilona Stepanyan

  3. Severtsov Institute of Ecology and Evolution, RAS, Moscow, Russia

    Eduard Galoyan

  4. Laboratório de Citogenética de Peixes, Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brazil

    Marcelo de Bello Cioffi

  5. Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Jena, Germany

    Marcelo de Bello Cioffi, Ahmed Al-Rikabi & Thomas Liehr

  6. Skolkovo Institute of Science and Technology, Moscow, Russia

    Elena Martynova

  7. Institute of Gene Biology, RAS, Moscow, Russia

    Irena Martirosyan

  8. Department of Zoology, Yerevan State University, Yerevan, Armenia

    Felix Danielyan & Marine Arakelyan

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  1. Victor Spangenberg
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  12. Marine Arakelyan
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Contributions

VS conceived of or designed the study. VS, AAR, TL, MA, IS, EM, and IM performed research. VS, IS, MBC, TG, FD, and OK analyzed the data. VS, MBC, AAR, and TL contributed to new methods. VS, OK, MA, MBC, IS, and EG wrote the paper.

Corresponding author

Correspondence to Victor Spangenberg.

Ethics declarations

The manipulations with the animals followed the international rules of the Manual on Humane Use of Animals in Biomedical Research. All experimental protocols were approved by the Ethics Committee for Animal Research of the Vavilov Institute of General Genetics (protocol No. 3, November 10, 2016) in accordance with the Regulations for Laboratory Practice.

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Spangenberg, V., Kolomiets, O., Stepanyan, I. et al. Evolution of the parthenogenetic rock lizard hybrid karyotype: Robertsonian translocation between two maternal chromosomes in Darevskia rostombekowi. Chromosoma 129, 275–283 (2020). https://doi.org/10.1007/s00412-020-00744-7

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