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Tracking the evolutionary pathway of sex chromosomes among fishes: characterizing the unique XX/XY1Y2 system in Hoplias malabaricus (Teleostei, Characiformes)

Abstract

The Neotropical fish, Hoplias malabaricus, is one of the most cytogenetically studied fish taxon with seven distinct karyomorphs (A–G) comprising varying degrees of sex chromosome differentiation, ranging from homomorphic to highly differentiated simple and multiple sex chromosomes. Therefore, this fish offers a unique opportunity to track evolutionary mechanisms standing behind the sex chromosome evolution and differentiation. Here, we focused on a high-resolution cytogenetic characterization of the unique XX/XY1Y2 multiple sex chromosome system found in one of its karyomorphs (G). For this, we applied a suite of conventional (Giemsa-staining, C-banding) and molecular cytogenetic approaches, including fluorescence in situ hybridization FISH (with 5S and 18S rDNAs, 10 microsatellite motifs and telomeric (TTAGGG) n sequences as probes), comparative genomic hybridization (CGH), and whole chromosome painting (WCP). In addition, we performed comparative analyses with other Erythrinidae species to discover the evolutionary origin of this unique karyomorph G-specific XY1Y2 multiple sex chromosome system. WCP experiments confirmed the homology between these multiple sex chromosomes and the nascent XX/XY sex system found in the karyomorph F, but disproved a homology with those of karyomorphs A–D and other closely related species. Besides, the putative origin of such XY1Y2 system by rearrangements of several chromosome pairs from an ancestral karyotype was also highlighted. In addition, clear identification of a male-specific region on the Y1 chromosome suggested a differential pattern of repetitive sequences accumulation. The present data suggested the origin of this unique XY1Y2 sex system, revealing evidences for the high level of plasticity of sex chromosome differentiation within the Erythrinidae.

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Acknowledgements

We would like to thank Mr. Valdivino Pereira da Silva (Renascer farm) for helping during the sampling process.

Funding

This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (Proc. Nos. 304992/2015-1, 306896/2014-1, and 152105/2016-6) and Fundação de Amparo à Pesquisa do Estado de São Paulo- FAPESP (Proc. No. 2016/21411-7) and further by the projects EXCELLENCE CZ.02.1.01/0.0/0.0/15_003/0000460 OP RDE and RVO: 67985904.

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Correspondence to Marcelo de Bello Cioffi.

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The experiments followed ethical and anesthesia conducts, in accordance with the Ethics Committee on Animal Experimentation of the Universidade Federal de São Carlos (Process Number CEUA 1853260315).

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The authors declare that they have no conflict of interest.

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Informed consent was obtained from all individual participants included in the study.

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Both Ezequiel Aguiar de Oliveira and Alexandr Sember will share the first authorship of this manuscript.

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de Oliveira, E.A., Sember, A., Bertollo, L.A.C. et al. Tracking the evolutionary pathway of sex chromosomes among fishes: characterizing the unique XX/XY1Y2 system in Hoplias malabaricus (Teleostei, Characiformes). Chromosoma 127, 115–128 (2018). https://doi.org/10.1007/s00412-017-0648-3

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  • DOI: https://doi.org/10.1007/s00412-017-0648-3

Keywords

  • Fish cytogenetics
  • Male-specific region
  • Whole chromosome painting
  • Comparative genomic hybridization
  • Repetitive sequences