A replication-time-controlling sequence element in Schizosaccharomyces pombe
Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome.
KeywordsARS elements Replication origins Replication timing 2D gel analysis Fission yeast
We are grateful to Mitradas M. Panicker, K. VijayRaghavan, Joel A. Huberman, and Rajiva Raman for extending laboratory facilities for conducting part of the work and to JAH for critical reading of the manuscript and valuable suggestions. This work was supported by the Council of Scientific and Industrial Research (CSIR), India, Research Grant # 38(1233)/09/EMRII to DDD. VPT was a recipient of a Senior Research Fellowship from the Indian Council of Medical Research (ICMR), India.
DDD conceived and coordinated the study. DDD and VPT designed and performed the experiments. DDD and VPT wrote the paper.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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