, Volume 126, Issue 4, pp 465–471 | Cite as

A replication-time-controlling sequence element in Schizosaccharomyces pombe

Original Article


Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome.


ARS elements Replication origins Replication timing 2D gel analysis Fission yeast 



We are grateful to Mitradas M. Panicker, K. VijayRaghavan, Joel A. Huberman, and Rajiva Raman for extending laboratory facilities for conducting part of the work and to JAH for critical reading of the manuscript and valuable suggestions. This work was supported by the Council of Scientific and Industrial Research (CSIR), India, Research Grant # 38(1233)/09/EMRII to DDD. VPT was a recipient of a Senior Research Fellowship from the Indian Council of Medical Research (ICMR), India.

Authors’ contributions

DDD conceived and coordinated the study. DDD and VPT designed and performed the experiments. DDD and VPT wrote the paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

412_2016_606_MOESM1_ESM.docx (14 kb)
Supplemental Table 1 (DOCX 14 kb)
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Supplemental Fig. 1

Diagram showing the strategy for confirmation of the new S. pombe strains by PCR. [Cont. = Chromosomal context; LF1 = Left flanking 1; Ura4 = Selectable marker; LF2 = Left flanking 2; ARS = ars2004/ars727; RF1 = Right flanking 1]. Amplification by the two pairs of primers, (i) context F-ura4R and (ii) ura4F-ARSF/R, confirms the new strains. (GIF 2881 kb)

412_2016_606_MOESM2_ESM.tif (2.8 mb)
High Resolution Image (TIFF 2881 kb)
412_2016_606_Fig6_ESM.gif (18.6 mb)
Supplemental Fig. 2

FACS profiles of different cell types at different sampling time points. (GIF 19079 kb)

412_2016_606_MOESM3_ESM.tif (18.6 mb)
High Resolution Image (TIFF 19079 kb)


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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of BiotechnologyVeer Bahadur Singh Purvanchal UniversityJaunpurIndia

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