Chromosoma

, Volume 126, Issue 4, pp 465–471 | Cite as

A replication-time-controlling sequence element in Schizosaccharomyces pombe

Original Article

Abstract

Eukaryotic replication origins are highly variable in their activity and replication timing. The nature and role of cis-acting regulatory sequences that control chromosomal replication timing is not well defined. In the fission yeast, Schizosaccharomyces pombe, a 200-bp late-replication-enforcing element (LRE), has been shown to enforce late replication of ARS elements in plasmids. Here, we show that a short (133-bp) fragment of the LRE (shLRE) is required for causing late replication of adjoining origins in its native as well as in an ectopic early-replicating chromosomal location. Active from both sides of an early-replicating origin, the shLRE is a bona fide cis-acting regulatory element that imposes late replication timing in the chromosome.

Keywords

ARS elements Replication origins Replication timing 2D gel analysis Fission yeast 

Supplementary material

412_2016_606_MOESM1_ESM.docx (14 kb)
Supplemental Table 1(DOCX 14 kb)
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Supplemental Fig. 1

Diagram showing the strategy for confirmation of the new S. pombe strains by PCR. [Cont. = Chromosomal context; LF1 = Left flanking 1; Ura4 = Selectable marker; LF2 = Left flanking 2; ARS = ars2004/ars727; RF1 = Right flanking 1]. Amplification by the two pairs of primers, (i) context F-ura4R and (ii) ura4F-ARSF/R, confirms the new strains. (GIF 2881 kb)

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High Resolution Image (TIFF 2881 kb)
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Supplemental Fig. 2

FACS profiles of different cell types at different sampling time points. (GIF 19079 kb)

412_2016_606_MOESM3_ESM.tif (18.6 mb)
High Resolution Image (TIFF 19079 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  1. 1.Department of BiotechnologyVeer Bahadur Singh Purvanchal UniversityJaunpurIndia

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