Chromosome identification for the carnivorous plant Genlisea margaretae
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Genlisea margaretae, subgenus Genlisea, section Recurvatae (184 Mbp/1C), belongs to a plant genus with a 25-fold genome size difference and an extreme genome plasticity. Its 19 chromosome pairs could be distinguished individually by an approach combining optimized probe pooling and consecutive rounds of multicolor fluorescence in situ hybridization (mcFISH) with bacterial artificial chromosomes (BACs) selected for repeat-free inserts. Fifty-one BACs were assigned to 18 chromosome pairs. They provide a tool for future assignment of genomic sequence contigs to distinct chromosomes as well as for identification of homeologous chromosome regions in other species of the carnivorous Lentibulariaceae family, and potentially of chromosome rearrangements, in cases where more than one BAC per chromosome pair was identified.
KeywordsGenlisea Multicolor fluorescence in situ hybridization (mcFISH) Reprobing BACs Karyotyping
We thank Ines Walde and Kristin Langanke for technical assistance; Tomáš Beseda, Jan Vrána, and Jana Dostálová for assistance with flow sorting and BAC library construction; and Giang T.H. Vu and Hieu X. Cao for helpful discussions. This work was supported by a grant of the Deutsche Forschungsgemeinschaft to IS and JF (SCHU 951/16-1), by the European Social Fund (CZ.1.07/2.3.00/20.0189) to IS, by the Ministry of Education, Youth and Sports of the Czech Republic (grant LO1204 from the National Program of Sustainability I) to HŠ and JD, and by a PhD scholarship of the Vietnam Ministry of Education and Training to TDT.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
The authors declare that they have no competing interests.
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