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Chromosoma

, Volume 120, Issue 3, pp 297–307 | Cite as

The Polycomb group protein CRAMPED is involved with TRF2 in the activation of the histone H1 gene

  • Jean-Michel GibertEmail author
  • François Karch
Research Article

Abstract

CRAMPED (CRM), conserved from plants to animals, was previously characterized genetically as a repressive factor involved in the formation of facultative and constitutive heterochromatin (Polycomb silencing, position effect variegation). We show that crm is dynamically regulated during replication and identify the Histone gene cluster (His-C) as a major CRM target. Surprisingly, CRM is specifically required for the expression of the Histone H1 gene, like the promoter-bound transcription factor TRF2. Consistently with this, CRM genetically interacts and co-immunoprecipitates with TRF2. However, the Polycomb phenotypes observed in crm mutants are not observed in TRF2 hypomorphic mutants, suggesting that they correspond to independent roles of CRM. CRM is thus a highly pleiotropic factor involved in both activation and repression.

Keywords

Salivary Gland TATA Binding Protein Polycomb Group Protein Position Effect Variegation PhiC31 Integrase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank all members of the Spierer, Pauli and Karch laboratories for stimulating discussions, in particular, Rob Maeda. We thank the Bloomington Drosophila Stock Center for the TRF2 G0039 and H2Av–RFP lines, Joseph Gall for the UAS–DLsm11–Venus line, Walter Gehring for the anti-CRM antibody, Frédérique Peronnet for the anti-PCNA antibody, and Robert Tjian for the anti-TRF2 antibody. We thank Mylène Docquier and Didier Chollet for their assistance at the genomic platform, Geneva.

Supplementary material

412_2011_312_Fig10_ESM.gif (143 kb)
Supplementary Fig 1

Expression of mCherry-CRM and nuclear GFP under the control of HS-Gal4 in salivary glands (GIF 143 kb) In the absence of heat shock, a homogenous expression of nuclear GFP is observed, whereas mCherry–CRM level is higher in some nuclei.

412_2011_312_MOESM1_ESM.tif (3.1 mb)
High resolution image (TIFF 3152 kb)

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Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Department of Zoology and Animal BiologyUniversity of GenevaGeneva 4Switzerland

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