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Calpain 2 is required for sister chromatid cohesion

Chromosoma volume 119, pages 267–274 (2010)Cite this article

Abstract

Calpains form a family of Ca2+-dependent cysteine proteases involved in diverse cellular processes. However, the specific functions of each calpain isoform remain unknown. Recent reports have shown that calpain 2 (Capn2) is essential for cell viability. We have recently shown that Capn2 is a nuclear protease associated with chromosomes during mitosis in mammalian embryonic cells. We now report that Capn2 depletion impairs mitosis and induces apoptosis in murine cells. Low Capn2 levels induce chromosome alignment defects, the loss of histone H3 threonine 3 phosphorylation at centromeres, and premature sister chromatid separation. Thus, Capn2 may play a role in fundamental mitotic functions, such as the maintenance of sister chromatid cohesion.

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Acknowledgments

We thank C. Johanet for providing the reagents and P. Coopman for the stimulating discussions. This work was supported by the Association Française contre les Myopathies (AFM), the Ligue Nationale Contre le Cancer (Comités Départementaux de l’Hérault and de l’Aveyron and Comité Régional du Languedoc-Roussillon), and the GEFLUC (Languedoc-Roussillon).

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Affiliations

  1. Cellular Motility Laboratory EPHE CNRS UMR 5539, University of Montpellier 2, Place E. Bataillon, 34095, Montpellier, France

    Yves Benyamin & Fabrice Raynaud

  2. CNRS UMR 6061, Université de Rennes 1, IFR 140, 2 Avenue du Pr Léon Bernard, 35043, Rennes, France

    Laura Magnaghi-Jaulin & Christian Jaulin

  3. Quantitative Cellular Biology Laboratory EPHE INSERM E343, University of Montpellier 2, Place E. Bataillon, 34095, Montpellier, France

    Mireille Rossel

  4. Department of Neuroscience, IGF, UMR 5203, 141 Rue Cardonille, 34094, Montpellier, France

    Fabrice Raynaud

  5. INSERM U710-UM2-EPHE, Université Montpellier 2, Place Eugene Bataillon-CC105, 34095, Montpellier Cedex 05, France

    Anne Marcilhac

Authors
  1. Laura Magnaghi-Jaulin
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  2. Anne Marcilhac
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  3. Mireille Rossel
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  4. Christian Jaulin
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  5. Yves Benyamin
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  6. Fabrice Raynaud
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Correspondence to Fabrice Raynaud.

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Magnaghi-Jaulin, L., Marcilhac, A., Rossel, M. et al. Calpain 2 is required for sister chromatid cohesion. Chromosoma 119, 267–274 (2010). https://doi.org/10.1007/s00412-010-0255-z

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  • DOI: https://doi.org/10.1007/s00412-010-0255-z

Keywords

  • Sister Chromatid
  • Nocodazole
  • Spindle Assembly Checkpoint
  • Mitotic Arrest
  • Calpain Inhibitor