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Chromosoma

, Volume 115, Issue 6, pp 427–436 | Cite as

Comparative genomic analysis links karyotypic evolution with genomic evolution in the Indian Muntjac (Muntiacus muntjak vaginalis)

  • Qi Zhou
  • Ling Huang
  • Jianguo Zhang
  • Xiangyi Zhao
  • Qingpeng Zhang
  • Fei Song
  • Jianxiang Chi
  • Fengtang YangEmail author
  • Wen WangEmail author
Research Article
  • 267 Downloads

Abstract

The karyotype of Indian muntjacs (Muntiacus muntjak vaginalis) has been greatly shaped by chromosomal fusion, which leads to its lowest diploid number among the extant known mammals. We present, here, comparative results based on draft sequences of 37 bacterial artificial clones (BAC) clones selected by chromosome painting for this special muntjac species. Sequence comparison on these BAC clones uncovered sequence syntenic relationships between the muntjac genome and those of other mammals. We found that the muntjac genome has peculiar features with respect to intron size and evolutionary rates of genes. Inspection of more than 80 pairs of orthologous introns from 15 genes reveals a significant reduction in intron size in the Indian muntjac compared to that of human, mouse, and dog. Evolutionary analysis using 19 genes indicates that the muntjac genes have evolved rapidly compared to other mammals. In addition, we identified and characterized sequence composition of the first BAC clone containing a chromosomal fusion site. Our results shed new light on the genome architecture of the Indian muntjac and suggest that chromosomal rearrangements have been accompanied by other salient genomic changes.

Keywords

Genome Size Chromosome Painting Draft Sequence Intron Size Fusion Site 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank Hongkun Zheng, Jun Li, Peixiang Ni, and Tao Feng in the Beijing Genomic Institute for assistance in preparing the perl scripts during the analysis. We also thank Huifeng Jiang, Yan Li, Yun Ding, Xin Li, and Haijing Yu in the Max-Planck Junior Research Group for incisive comments and discussions for preparing the manuscript and Xuebing Qi in the Comparative Genomics Group in Kunming Institute of Zoology for suggestions on performing PAML. This research was supported by key project grants of the Key Laboratory of Cellular and Molecular Evolution in Kunming Institute of Zoology and the National Natural Science Foundation of China (No. 30170506).

Supplementary material

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Qi Zhou
    • 1
    • 3
  • Ling Huang
    • 2
    • 3
  • Jianguo Zhang
    • 4
  • Xiangyi Zhao
    • 1
  • Qingpeng Zhang
    • 4
  • Fei Song
    • 4
  • Jianxiang Chi
    • 2
    • 3
  • Fengtang Yang
    • 2
    • 5
    Email author
  • Wen Wang
    • 1
    Email author
  1. 1.CAS-Max Planck Junior Research Group, Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of ZoologyChinese Academy of SciencesKunmingPeople’s Republic of China
  2. 2.Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of ZoologyChinese Academy of SciencesKunmingPeople’s Republic of China
  3. 3.Graduate School of Chinese Academy SciencesBeijingPeople’s Republic of China
  4. 4.Beijing Institute of GenomicsChinese Academy of SciencesBeijingPeople’s Republic of China
  5. 5.Wellcome Trust Sanger InstituteCambridgeUK

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