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New families of site-specific repetitive DNA sequences that comprise constitutive heterochromatin of the Syrian hamster (Mesocricetus auratus, Cricetinae, Rodentia)

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Abstract

We molecularly cloned new families of site-specific repetitive DNA sequences from BglII- and EcoRI-digested genomic DNA of the Syrian hamster (Mesocricetus auratus, Cricetrinae, Rodentia) and characterized them by chromosome in situ hybridization and filter hybridization. They were classified into six different types of repetitive DNA sequence families according to chromosomal distribution and genome organization. The hybridization patterns of the sequences were consistent with the distribution of C-positive bands and/or Hoechst-stained heterochromatin. The centromeric major satellite DNA and sex chromosome-specific and telomeric region-specific repetitive sequences were conserved in the same genus (Mesocricetus) but divergent in different genera. The chromosome-2-specific sequence was conserved in two genera, Mesocricetus and Cricetulus, and a low copy number of repetitive sequences on the heterochromatic chromosome arms were conserved in the subfamily Cricetinae but not in the subfamily Calomyscinae. By contrast, the other type of repetitive sequences on the heterochromatic chromosome arms, which had sequence similarities to a LINE sequence of rodents, was conserved through the three subfamilies, Cricetinae, Calomyscinae and Murinae. The nucleotide divergence of the repetitive sequences of heterochromatin was well correlated with the phylogenetic relationships of the Cricetinae species, and each sequence has been independently amplified and diverged in the same genome.

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Acknowledgements

This study was supported by a Grant-in-Aid for Scientific Research (No. 16086201) from the Ministry of Education, Culture, Sports, Science and Technology in Japan.

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Authors and Affiliations

  1. Laboratory of Animal Cytogenetics, Creative Research Initiative “Sousei”, Hokkaido University, North10 West 8, Kita-ku, Sapporo, 060-0810, Japan

    Kazuhiko Yamada, Eikichi Kamimura, Mariko Kondo, Chizuko Nishida-Umehara & Yoichi Matsuda

  2. Laboratory Animal Center, Yamagata University School of Medicine, Yamagata, 990-9585, Japan

    Eikichi Kamimura

  3. Laboratory of Wild Animals, Department of Zootechnical Sciences, Faculty of Agriculture, Tokyo University of Agriculture, Atsugi, 234-0034, Japan

    Kimiyuki Tsuchiya

  4. Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, 060-0810, Japan

    Chizuko Nishida-Umehara & Yoichi Matsuda

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  1. Kazuhiko Yamada
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  2. Eikichi Kamimura
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  3. Mariko Kondo
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  4. Kimiyuki Tsuchiya
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  5. Chizuko Nishida-Umehara
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  6. Yoichi Matsuda
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Correspondence to Yoichi Matsuda.

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Communicated by Y. Hiraoka

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Yamada, K., Kamimura, E., Kondo, M. et al. New families of site-specific repetitive DNA sequences that comprise constitutive heterochromatin of the Syrian hamster (Mesocricetus auratus, Cricetinae, Rodentia). Chromosoma 115, 36–49 (2006). https://doi.org/10.1007/s00412-005-0012-x

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  • DOI: https://doi.org/10.1007/s00412-005-0012-x

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