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TopBP1 localises to centrosomes in mitosis and to chromosome cores in meiosis

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Abstract

Topoisomerase IIβ binding protein 1 (TopBP1), previously shown to localise to sites of DNA damage and to stalled replication forks, has been implicated in DNA replication and in DNA damage response. In this work we showed that TopBP1 was localised in structures other than stalled replication forks. In late mitosis TopBP1 localises to centrosomes in a manner similar to other DNA damage response proteins such as BRCA1 and p53. Spindle checkpoint activation does not affect this centrosomal localisation. Moreover, in the testis, we detected high levels of TopBP1 associated with meiotic prophase chromosome cores and the X–Y pair. Together, these data suggest a direct role of TopBP1 during both mitosis and meiotic prophase I.

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Acknowledgements

This work was funded by grants from the Academy of Finland and the Cancer Society of Finland. R.F. is supported by FIS (Fondo de Investigaciones Sanitarias) (01/1624) and FUNCIS (Fundación Canaria de Investigación Sanitaria) (PI 45/00) grants. D.P. is supported by a predoctoral fellowship from the Spanish Ministry of Education and Culture. P.B.M is supported by NSERC of Canada. We thank M. Kallio for helpful advice, and H. Pospiech and D. Kaska for editing the manuscript.

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Authors and Affiliations

  1. Biocenter Oulu and Department of Biochemistry, P.O. Box 3000, 90014, University of Oulu, Finland

    Kaarina Reini, Lahja Uitto & Juhani E. Syväoja

  2. Hospital Universitario de Canarias, Unidad de investigación, Ofra s/n, La Cuesta, 38320, Tenerife, Spain

    David Perera & Raimundo Freire

  3. Department of Biology, York University, Downsview, Ontario, M3J 1P3, Canada

    Peter B. Moens

  4. Department of Biology, University of Joensuu, P.O. Box 111, 80101, Joensuu, Finland

    Juhani E. Syväoja

Authors
  1. Kaarina Reini
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  2. Lahja Uitto
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  5. Raimundo Freire
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  6. Juhani E. Syväoja
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Correspondence to Juhani E. Syväoja.

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Communicated by T. Hassold

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Reini, K., Uitto, L., Perera, D. et al. TopBP1 localises to centrosomes in mitosis and to chromosome cores in meiosis. Chromosoma 112, 323–330 (2004). https://doi.org/10.1007/s00412-004-0277-5

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