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Radiation and Environmental Biophysics

, Volume 40, Issue 3, pp 213–219 | Cite as

Micro-organisation of the membrane after radiation-induced apoptosis: a flow cytometry study

  • L. Vincent-Genod
  • M. Benderitter
  • P. Voisin
Original Paper

Abstract  

The aim of this study was to detect membrane fluidity modifications in blood lymphocytes that had been exposed to γ-radiation, at a graded series of depths from the surface to the centre of the membrane bilayer and as a function of cell viability. A time course was performed to verify the contribution of the membrane to radiation-induced apoptosis. In comparison with spectrofluorimetry, flow cytometry proved to be a reliable method for measuring radiation-induced membrane alterations. Late apoptotic lymphocytes were characterised by a significant decrease of the 3-SA, 6-SA and 9-SA fluorescence anisotropy values, compared to viable lymphocytes. Moreover, a highly significant difference was observed in the early apoptotic lymphocyte subpopulation between the fluorescence anisotropy values measured 24 h (radiation-induced apoptosis) and those measured 1 h (spontaneous apoptosis) after irradiation. The simultaneous assessment of cellular viability and membrane fluidity using n-(9-anthroyloxy) fatty acid probes, may be relevant for the investigation of interactions which may exist between membrane modifications and the apoptotic process. Our observations support the specificity of radiation-induced apoptosis compared to spontaneous apoptosis in terms of biophysical modifications of membrane properties.

Keywords

Flow Cytometry Reliable Method Blood Lymphocyte Membrane Fluidity Apoptotic Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • L. Vincent-Genod
    • 1
  • M. Benderitter
    • 1
  • P. Voisin
    • 1
  1. 1.Institut de Protection et de Sûreté Nucléaire (IPSN), Département de Protection de la Santé de l’Homme et de Dosimétrie, Section Autonome de Radiobiologie Appliquée à la Médecine, Laboratoire de Dosimétrie Biologique Multiparamétrique, BP 6, 92265 Fontenay-aux-Roses cedex, France e-mail: philippe.voisin@ipsn.fr Tel.: +33-1-46549550, Fax: +33-1-46548467FR

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