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Radiation and Environmental Biophysics

, Volume 40, Issue 2, pp 163–167 | Cite as

Bowman-Birk proteinase inhibitor-mediated radioprotection against UV irradiation is TP53-dependent and associated with stimulation of nucleotide excision repair

  • Klaus Dittmann
  • Daniele Knaus-Dittmann
  • Claus Mayer
  • H. P. Rodemann
Original Paper

Abstract 

The Bowman-Birk proteinase inhibitor (BBI) has previously been described as a radioprotective agent against ionising radiation. It was demonstrated that BBI is able to significantly increase the clonogenic cell survival of normal fibroblasts when applied before exposure to ultraviolet B (UVB) radiation. In transformed TP53-mutated cell lines, however, the BBI-mediated radioprotection was absent. At the molecular level, the radioprotective effect of BBI can be correlated with BBI-mediated stabilisation of TP53 protein prior to irradiation. Following UVB irradiation, the BBI-treated cells present an accelerated removal of cyclobutane pyrimidine dimers. Thus, the cell and molecular biological data presented suggest that BBI is able to protect cells with functional TP53 from UVB-induced DNA damage. This protective effect is most likely achieved via the activation of the TP53 signalling cascade resulting in the activation of nucleotide excision repair.

Keywords

Nucleotide Excision Repair Normal Fibroblast Pyrimidine Dimer Clonogenic Cell Cyclobutane Pyrimidine Dimer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Klaus Dittmann
    • 1
  • Daniele Knaus-Dittmann
    • 1
  • Claus Mayer
    • 1
  • H. P. Rodemann
    • 1
  1. 1.Section of Radiobiology and Molecular Environmental Research, Department of Radiotherapy, Eberhard-Karls-University, Röntgenweg 11, 72076 Tübingen, Germany e-mail: hans-peter.rodemann@uni-tuebingen.de Tel.: +49-7071-2985862, Fax: +49-7071-295900DE

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