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Radiation and Environmental Biophysics

, Volume 37, Issue 2, pp 101–106 | Cite as

Formation of plasmid DNA strand breaks induced by low-energy ion beam: indication of nuclear stopping effects

  • Y. Chen
  • Bingyao Jiang
  • Youshan Chen
  • Xingzhao Ding
  • Xianghuai Liu
  • Ceshi Chen
  • Xinyou Guo
  • Guanglin Yin
ORIGINAL PAPER

Abstract

Plasmid pGEM 3zf(+) was irradiated by nitrogen ion beam with energies between 20 and 100 keV and the fluence kept as 1×1012 ions/cm2. The irradiated plasmid was assayed by neutral electrophoresis and quantified by densitometry. The yields of DNA with single-strand and double-strand breaks first increased then decreased with increasing ion energy. There was a maximal yield value in the range of 20–100 keV. The relationship between DNA double-strand breaks (DSB) cross-section and linear energy transfer (LET) also showed a peak-shaped distribution. To understand the physical process during DNA strand breaks, a Monte Carlo calculation code known as TRIM (Transport of Ions in Matter) was used to simulate energy losses due to nuclear stopping and to electronic stopping. It can be assumed that nuclear stopping plays a more important role in DNA strand breaks than electronic stopping in this energy range. The physical mechanisms of DNA strand breaks induced by a low-energy ion beam are also discussed.

Keywords

Calculation Code Linear Energy Transfer Monte Carlo Calculation Plasmid pGEM Irradiate Plasmid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Y. Chen
    • 1
  • Bingyao Jiang
    • 1
  • Youshan Chen
    • 1
  • Xingzhao Ding
    • 1
  • Xianghuai Liu
    • 1
  • Ceshi Chen
    • 2
  • Xinyou Guo
    • 2
  • Guanglin Yin
    • 2
  1. 1.Ion Beam Laboratory, Shanghai Institute of Metallurgy, Chinese Academy of Sciences, Shanghai 200050, P. R. ChinaCN
  2. 2.Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai 200233, P. R. ChinaCN

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