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Mutations and chromosomal aberrations in hMTH1-transfected and non-transfected TK6 cells after exposure to low dose rates of gamma radiation

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Abstract

The aim of the present study was to analyse the dose rate effect of gamma radiation at the level of mutations, chromosomal aberrations, and cell growth in TK6 cells with normal as well as reduced levels of hMTH1 protein. TK6 cells were exposed to gamma radiation at dose rates ranging from 1.4 to 30.0 mGy/h (chronic exposure) as well as 24 Gy/h (acute exposure). Cell growth, frequency of thymidine kinase mutants, and of chromosomal aberrations in painted chromosomes 2, 8, and 14 were analysed. A decline in cell growth and an increase in unstable-type chromosomal aberrations with increasing dose rate were observed in both cell lines. A dose rate effect was not seen on mutations or stable-type chromosomal aberrations in any of the two cell lines. Reduction in the hMTH1 protein does not influence the sensitivity of TK6 cells to gamma radiation. This result fits well with data of others generated with the same cell line.

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Acknowledgments

The study was supported by a grant from the Swedish Radiation Safety Authority (SSM).

Conflict of interest

The authors declare that they have no conflict of interest.

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Author notes

  1. Marta Deperas-Kaminska

    Present address: Department of Medical Genetics, Institute of Mother and Child, Warsaw, Poland

Authors and Affiliations

  1. Department of Molecular Biosciences, Centre for Radiation Protection Research (CRPR), The Wenner-Gren Institute, Stockholm University, 106 91, Stockholm, Sweden

    Sara Shakeri Manesh, Marta Deperas-Kaminska, Asal Fotouhi, Traimate Sangsuwan, Mats Harms-Ringdahl, Andrzej Wojcik & Siamak Haghdoost

  2. Institute of Biology, Jan Kochanowski University, Kielce, Poland

    Andrzej Wojcik

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  1. Sara Shakeri Manesh
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  2. Marta Deperas-Kaminska
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Correspondence to Siamak Haghdoost.

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Shakeri Manesh, S., Deperas-Kaminska, M., Fotouhi, A. et al. Mutations and chromosomal aberrations in hMTH1-transfected and non-transfected TK6 cells after exposure to low dose rates of gamma radiation. Radiat Environ Biophys 53, 417–425 (2014). https://doi.org/10.1007/s00411-014-0521-1

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