Radiation and Environmental Biophysics

, Volume 52, Issue 3, pp 411–417 | Cite as

UV microspot irradiator at Columbia University

  • Alan W. BigelowEmail author
  • Brian Ponnaiya
  • Kimara L. Targoff
  • David J. Brenner
Short Communication


The Radiological Research Accelerator Facility at Columbia University has recently added a UV microspot irradiator to a microbeam irradiation platform. This UV microspot irradiator applies multiphoton excitation at the focal point of an incident laser as the source for cell damage, and with this approach, a single cell within a 3D sample can be targeted and exposed to damaging UV. The UV microspot’s ability to impart cellular damage within 3D is an advantage over all other microbeam techniques, which instead impart damage to numerous cells along microbeam tracks. This short communication is an overview, and a description of the UV microspot including the following applications and demonstrations of selective damage to live single cell targets: DNA damage foci formation, patterned irradiation, photoactivation, targeting of mitochondria, and targeting of individual cardiomyocytes in a live zebrafish embryo.


DNA damage Microbeams UV 



The authors thank: Asithambi Aroumougame from the David Chen group at UTSW for providing GFP-tagged XRCC1 HT1080 cells and EGFP-tagged OGG1 HT1080 cells; Iris Muller at GSI and Jonathan Chubb at the University of Dundee for providing a triple-tagged HT1080 cell line (RFP, YFP, and photoactivable GFP); and Michael Kruhlak at NIH/NCI for providing HeLa cells with photoactivable GFP. The authors also thank Columbia University employees Helen Turner for preparing and handling HT1080 cells, Vanessa George and Manuela Buonanno for preparing and handling the zebrafish embryo samples, and Gary Johnson for instrumentation development. This work was supported by the National Institute of Biomedical Imaging and Bioengineering under Grant: NIBIB 5P41 EB002033-16.


  1. Asaithamby A, Chen DJ (2011) Mechanism of cluster DNA damage repair in response to high-atomic number and energy particles radiation. Mutat Res Fundam Mol Mech Mutagen 711(1–2):87–99CrossRefGoogle Scholar
  2. Belyakov OV, Mitchell SA, Parikh D, Randers-Pehrson G, Marino SA, Amundson SA, Geard CR, Brenner DJ (2005) Biological effects in unirradiated human tissue induced by radiation damage up to 1 mm away. Proc Natl Acad Sci USA 102(40):14203–14208ADSCrossRefGoogle Scholar
  3. Bertucci A, Pocock RD, Randers-Pehrson G, Brenner DJ (2009) Microbeam irradiation of the C. elegans nematode. J Radiat Res 50(Suppl A):A49–A54CrossRefGoogle Scholar
  4. Bigelow AW, Geard CR, Randers-Pehrson G, Brenner DJ (2008) Microbeam-integrated multiphoton imaging system. Rev Sci Instrum 79(12):123707ADSCrossRefGoogle Scholar
  5. Bigelow AW, Randers-Pehrson G, Garty G, Geard CR, Xu Y, Harken AD, Johnson GW, Brenner DJ (2011) Ion, X-ray, UV and neutron microbeam systems for cell irradiation. AIP Conf Proc 1336(1):351–355ADSCrossRefGoogle Scholar
  6. Harken AD, Randers-Pehrson G, Johnson GW, Brenner DJ (2011) The Columbia University proton-induced soft X-ray microbeam. Nucl Instrum Method Phys Res Sect B 269(18):1992–1996ADSCrossRefGoogle Scholar
  7. Mably JD, Burns CG, Chen J-N, Fishman MC, Mohideen M-APK (2003) Heart of glass regulates the concentric growth of the heart in zebrafish. Curr Biol 13(24):2138–2147CrossRefGoogle Scholar
  8. Xu Y, Randers-Pehrson G, Marino SA, Bigelow AW, Akselrod MS, Sykora JG, Brenner DJ (2011) An accelerator-based neutron microbeam system for studies of radiation effects. Radiat Prot Dosim 145(4):373–376CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Alan W. Bigelow
    • 1
    Email author
  • Brian Ponnaiya
    • 1
  • Kimara L. Targoff
    • 2
  • David J. Brenner
    • 1
  1. 1.Center for Radiological ResearchColumbia University Medical CenterNew YorkUSA
  2. 2.Division of Pediatric Cardiology, Department of Pediatrics, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

Personalised recommendations