Abstract
For radiation exposures employing targeted sources such as particle microbeams, the deposition of energy and dose will depend on the spatial heterogeneity of the sample. Although cell structural variations are relatively minor for two-dimensional cell cultures, they can vary significantly for fully differentiated tissues. Employing high-resolution confocal microscopy, we have determined the spatial distribution, size, and shape of epidermal keratinocyte nuclei for the full-thickness EpiDerm™ skin model (MatTek, Ashland, VA). Application of these data to calculate the microdosimetry and microdistribution of energy deposition by an electron microbeam is discussed.
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Acknowledgments
This work was supported by the Biological and Environmental Research Program (BER), US Department of Energy under contract DE-AC05-76RLO1830.
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This paper is based on a presentation made at the 9th International Microbeam Workshop, July 15–17, 2010, in Darmstadt, Germany.
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Miller, J.H., Chrisler, W.B., Wang, X. et al. Confocal microscopy for modeling electron microbeam irradiation of skin. Radiat Environ Biophys 50, 365–369 (2011). https://doi.org/10.1007/s00411-011-0371-z
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DOI: https://doi.org/10.1007/s00411-011-0371-z