Radiation and Environmental Biophysics

, Volume 46, Issue 4, pp 395–400 | Cite as

Demonstration of a radiation-induced bystander effect for low dose low LET β-particles

  • Rudranath Persaud
  • Hongning Zhou
  • Tom K. Hei
  • Eric J. HallEmail author
Original Paper


Radiation-induced bystander mutagenesis at a relatively low dose range was investigated using low LET β-particles in a three-dimensional cell culture model. CHO cells were labeled with 0, 0.5, 1.0 or 5.0 μCi tritiated thymidine (3HdTTP) for 12 h and subsequently incubated with AL cells for 24 h at 11°C. The cell mixture was centrifuged to produce a spheroid of 4 × 106 cells of which there was five times more AL than CHO cells. The short-range β-particles emitted by 3HdTTP result in self-irradiation of labeled CHO cells, thus biological effects on neighboring AL cells can be attributed to the bystander response. To evaluate such response, non-labeled bystander AL cells were isolated from among labeled CHO cells and studied independently for survival and mutagenesis. Treatment of CHO cells with 3HdTTP resulted in a dose-dependent increase in bystander mutation incidence among neighboring AL cells compared to controls. In addition, multiplex PCR analysis revealed the types of mutants to be significantly different from those of spontaneous origin. These data provide evidence that low dose low LET radiation can induce bystander mutagenesis in a three-dimensional model. The results of this study will address the relevant issues of actual target size and radiation quality, and are likely to have a significant impact on our current understanding of radiation risk assessment.


Chinese Hamster Ovary Cell Chinese Hamster Ovary Bystander Effect Tritiated Thymidine Clonogenic Survival 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was supported by grants from the US Department of Energy DE-FG02-03ER63441 of the Low Dose Program and the National Institutes of Health CA 49062 and ES 11804.


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Rudranath Persaud
    • 1
  • Hongning Zhou
    • 1
  • Tom K. Hei
    • 1
  • Eric J. Hall
    • 1
    Email author
  1. 1.Center for Radiological ResearchColumbia University Medical Center New YorkUSA

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