Radiation and Environmental Biophysics

, Volume 45, Issue 2, pp 93–98 | Cite as

The effect of the β-emitting yttrium-90 citrate on the dose–response of dicentric chromosomes in human lymphocytes: a basis for biological dosimetry after radiosynoviorthesis

  • E. SchmidEmail author
  • H.-J. Selbach
  • M. Voth
  • J. Pinkert
  • F. J. Gildehaus
  • R. Klett
  • M. Haney
Original Paper


The production of dicentric chromosomes in human lymphocytes by β-particles of yttrium-90 (Y-90) was studied in vitro to provide a basis of biological dosimetry after radiosynoviorthesis (RSO) of persistent synovitis by intra-articular administration of yttrium-90 citrate colloid. Since the injected colloid may leak into the lymphatic drainage exposing other parts of the body to radiation, the measurement of biological damage induced by β-particles of Y-90 is important for the assessment of radiation risk to the patients. A linear dose–response relationship (α = 0.0229 ± 0.0028 dicentric chromosomes per cell per gray) was found over the dose range of 0.2176–2.176 Gy. The absorbed doses were calculated for exposure of blood samples to Y-90 activities from 40 to 400 kBq using both Monte Carlo simulation and an analytical model. The maximum low-dose RBE, the RBEM which is equivalent to the ratio of the α coefficients of the dose–response curves, is well in line with published results obtained earlier for irradiation of blood of the same donor with heavily filtered 220 kV X-rays (3.35 mm copper), but half of the RBEM relative to weakly filtered 220 kV X-rays. Therefore, it can be concluded that for estimating an absorbed dose during RSO by the technique of biological dosimetry, in vitro and in vivo data for the same radiation quality are necessary.


Human Lymphocyte Relative Biological Effectiveness Track Length Tritiated Water Monte Carlo Code 
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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • E. Schmid
    • 1
    Email author
  • H.-J. Selbach
    • 2
  • M. Voth
    • 3
  • J. Pinkert
    • 4
  • F. J. Gildehaus
    • 5
  • R. Klett
    • 6
  • M. Haney
    • 7
  1. 1.Radiobiological InstituteUniversity of MunichMunichGermany
  2. 2.Department 6.2Physikalisch-Technische Bundesanstalt (PTB)BraunschweigGermany
  3. 3.Schering AGBerlinGermany
  4. 4.Schering Deutschland GmbHBerlinGermany
  5. 5.Institute of Nuclear MedicineUniversity of MunichMunichGermany
  6. 6.Department of Nuclear MedicineUniversity Hospital of Giessen and MarburgGiessenGermany
  7. 7.Institute of RadiobiologyGSF-National Research Center for Environment and HealthNeuherbergGermany

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