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Radiation and Environmental Biophysics

, Volume 44, Issue 3, pp 219–224 | Cite as

Space radiation does not induce a significant increase of intrachromosomal exchanges in astronauts’ lymphocytes

  • M. Horstmann
  • M. DuranteEmail author
  • C. Johannes
  • R. Pieper
  • G. Obe
Original Paper

Abstract

Chromosome aberration analysis in astronauts has been used to provide direct, biologically motivated estimates of equivalent doses and risk associated to cosmic radiation exposure during space flight. However, the past studies concentrated on measurements of dicentrics and translocations, while chromosome intrachanges (inversions) have never been measured in astronauts’ samples. Recent data reported in the literature suggest that densely ionizing radiation can induce a large fraction of intrachanges, thus leading to the suspicion that interchanges grossly underestimate the cosmic radiation-induced cytogenetic damage in astronauts. We have analyzed peripheral blood lymphocytes from 11 astronauts involved in short- or long-term space flights in low-Earth orbit using high-resolution multicolor banding to assess the frequency of intrachromosomal exchanges in both pre- and post-flight samples. We did not detect any inversions in chromosome 5 from a total of 2800 cells in astronauts’ blood. In addition, no complex type exchanges were found in a total of 3590 astronauts’ lymphocytes analyzed by multifluor fluorescence in situ hybridisation. We conclude that, within the statistical power of this study, the analysis of interchanges for biological dosimetry in astronauts does not significantly underestimate the space radiation-induced cytogenetic damage, and complex-type exchanges or intrachanges have limited practical use for biodosimetry at very low doses.

Keywords

Plutonium Chromosomal Aberration International Space Station Space Flight Space Radiation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

This work was funded by the German Space Agency (DLR) Grant 50WB0030.

References

  1. 1.
    NCRP (2000) Radiation protection guidance for activities in low-Earth orbit. NCRP Report No. 132, Bethesda, MarylandGoogle Scholar
  2. 2.
    Cucinotta FA, Schimmerling W, Wilson JW, Peterson LE, Saganti PB, Dicello JF (2004) Uncertainties in estimates of the risks of late effects from space radiation. Adv Space Res 34:1383–1389CrossRefPubMedADSGoogle Scholar
  3. 3.
    NCRP (2002) Operational radiation safety program for astronauts in low Earth orbit: a Basic Framework. NCRP Report No. 142, Bethesda, MarylandGoogle Scholar
  4. 4.
    Testard I, Ricoul M, Hoffschir F, Flury-Herard A, Dutrillaux B, Fedorenko B, Gerasimenko V, Sabatier L (1996) Radiation-induced chromosome damage in astronauts’ lymphocytes. Int J Radiat Biol 70:403–411CrossRefPubMedGoogle Scholar
  5. 5.
    Obe G, Johannes I, Johannes C, Hallman K, Reitz G, Facius R (1997) Chromosomal aberrations in blood lymphocytes of astronauts after long-term space flights. Int J Radiat Biol 72:727–734CrossRefPubMedGoogle Scholar
  6. 6.
    Yang TC, George K, Johnson AS, Durante M, Fedorenko BS (1997) Biodosimetry results from space flight Mir-18. Radiat Res 148:S17–S23PubMedCrossRefGoogle Scholar
  7. 7.
    Testard I, Sabatier L (1999) Biological dosimetry for astronauts: a real challenge. Mutat Res 430:315–326PubMedGoogle Scholar
  8. 8.
    Fedorenko B, Druzhinin S, Yudaeva L, Petrov V, Akatov Y, Snigiryova G, Novitskaya N, Shevchenko V, Rubanovich A (2001) Cytogenetic studies of blood lymphocytes from cosmonauts after long-term space flights on Mir station. Adv Space Res 27:355–359CrossRefPubMedADSGoogle Scholar
  9. 9.
    George K, Durante M, Wu HL, Willingham V, Badhwar G, Cucinotta FA (2001) Chromosome aberrations in the blood lymphocytes of astronauts after space flight. Radiat Res 156:731–738PubMedCrossRefGoogle Scholar
  10. 10.
    George K, Durante M, Willingham V, Cucinotta FA (2004) Chromosome aberrations of clonal origin are present in astronauts’ blood lymphocytes. Cytogenet Genome Res 104:245–251CrossRefPubMedGoogle Scholar
  11. 11.
    Greco O, Durante M, Gialanella G, Grossi G, Pugliese M, Scampoli P, Snigiryova G, Obe G (2003) Biological dosimetry in Russian and Italian astronauts. Adv Space Res 31:1495–1503CrossRefPubMedADSGoogle Scholar
  12. 12.
    Durante M, Snigiryova G, Akaeva E, Bogomazova A, Druzhinin S, Fedorenko B, Greco O, Novitskaya N, Rubanovich A, Shevchenko V, Von Recklinghausen U, Obe G (2003) Chromosome aberration dosimetry in cosmonauts after single or multiple space flights. Cytogenet Genome Res 103:40–46CrossRefPubMedGoogle Scholar
  13. 13.
    Durante M, Bonassi S, George K, Cucinotta FA (2001) Risk estimation based on chromosomal aberrations induced by radiation. Radiat Res 156:662–667PubMedCrossRefGoogle Scholar
  14. 14.
    Hande MP, Azizova TV, Geard CR, Burak LE, Mitchell CR, Khokhryakov VF, Vasilenko EK, Brenner DJ (2003) Past exposure to densely ionizing radiation leaves a unique permanent signature in the genome. Am J Hum Genet 72:1162–1170CrossRefPubMedGoogle Scholar
  15. 15.
    Mitchell CR, Azizova TV, Hande MP, Burak LE, Tsakok JM, Khokhryakov VF, Geard CR, Brenner DJ (2004) Stable intrachromosomal biomarkers of past exposure to densely ionizing radiation in several chromosomes of exposed individuals. Radiat Res 162:257–263PubMedCrossRefGoogle Scholar
  16. 16.
    Cucinotta FA, Wilson JW, Shinn JL, Badavi FF, Badhwar GD (1996) Effects of target fragmentation on evaluation of LET spectra from space radiations: implications for space radiation protection studies. Radiat Meas 26:923–934CrossRefPubMedGoogle Scholar
  17. 17.
    Cucinotta FA, Wilson JW, Williams JR, Dicello JF (2000) Analysis of MIR-18 results for physical and biological dosimetry: radiation shielding effectiveness in LEO. Radiat Meas 32:181–191CrossRefPubMedGoogle Scholar
  18. 18.
    Obe G, Facius R, Reitz G, Johannes I, Johannes C (1999) Manned missions to Mars and chromosome damage. Int J Radiat Biol 75:429–433CrossRefPubMedGoogle Scholar
  19. 19.
    Chudoba I, Hickmann G, Friedrich T, Jauch A, Kozlowski P, Senger G (2004) mBAND: a high resolution multicolor banding technique for the detection of complex intrachromosomal aberrations. Cytogenet Genome Res 104:390–393CrossRefPubMedGoogle Scholar
  20. 20.
    Badhwar GD, Atwell W, Reitz G, Beaujean R, Heinrich W (2002) Radiation measurements on the Mir Orbital Station. Radiat Meas 35:393–422CrossRefPubMedGoogle Scholar
  21. 21.
    Johannes C, Horstmann M, Durante M, Chudoba I, Obe G (2004) Chromosome intrachanges and interchanges detected by multicolor banding in lymphocytes: searching for clastogen signatures in the human genome. Radiat Res 161:540–548PubMedCrossRefGoogle Scholar
  22. 22.
    Horstmann M, Durante M, Obe G (2004) Distribution of breakpoints and fragment sizes in human chromosome 5 after heavy-ion bombardment. Int J Radiat Biol 80:437–443CrossRefPubMedGoogle Scholar
  23. 23.
    Cornforth MN (2001) Analyzing radiation-induced complex chromosome rearrangements by combinatorial painting. Radiat Res 155:643–659PubMedCrossRefGoogle Scholar
  24. 24.
    Horstmann M, Obe G (2003) CABAND: Classification of aberrations in multicolor banded chromosomes. Cytogenet Genome Res 103:24–27CrossRefPubMedGoogle Scholar
  25. 25.
    Anderson RM, Tsepenko VV, Gasteva GN, Molokanov AA, Sevan’kaev AV, Goodhead DT (2005) mFISH analysis reveals complexity of chromosome aberrations in individuals occupationally exposed to internal plutonium: a pilot study to assess the relevance of complex aberrations as biomarkers of exposure to high-LET alpha particles. Radiat Res 163:26–35PubMedCrossRefGoogle Scholar
  26. 26.
    Hande MP, Azizova TV, Burak LE, Khokhryakov VF, Geard CR, Brenner DJ (2005) Complex chromosome aberrations persist in individuals many years after occupational exposure to densely ionizing radiation: an mFISH study. Genes Chromosomes Cancer 44:1–9CrossRefPubMedGoogle Scholar
  27. 27.
    Horstmann M, Durante M, Johannes C, Obe G (2005) Chromosomal intrachanges induced by swift iron ions. Adv Space Res 35:276–279CrossRefPubMedADSGoogle Scholar
  28. 28.
    Durante M, George K, Wu HL, Cucinotta FA (2002) Karyotypes of human lymphocytes exposed to high-energy iron ions. Radiat Res 158:581–590PubMedCrossRefGoogle Scholar
  29. 29.
    Durante M, Ando K, Furusawa Y, Obe G, George K, Cucinotta FA (2004) Complex chromosomal rearrangements induced in vivo by heavy ions. Cytogenet Genome Res 104:240–244CrossRefPubMedGoogle Scholar
  30. 30.
    Tawn EJ, Whitehouse CA (2005) Chromosome intra- and inter-changes determined by G-banding in radiation workers with in vivo exposure to plutonium. J Radiol Prot 25:83–88CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. Horstmann
    • 2
  • M. Durante
    • 1
    Email author
  • C. Johannes
    • 2
  • R. Pieper
    • 2
  • G. Obe
    • 2
  1. 1.Department of PhysicsUniversity Federico IINapoliItaly
  2. 2.Department of GeneticsUniversity of Duisburg-EssenEssenGermany

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