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Lung

, Volume 194, Issue 1, pp 67–74 | Cite as

Delayed Administration of WP1066, an STAT3 Inhibitor, Ameliorates Radiation-Induced Lung Injury in Mice

  • Jiahua YuEmail author
  • Xiaopeng Yuan
  • Yang Liu
  • Kaishuo Zhang
  • Jie Wang
  • Haowen Zhang
  • Fenju LiuEmail author
Article

Abstract

Purpose

The present study was designed to investigate the effects of WP1066, a specific inhibitor of STAT3 signaling, on radiation-induced lung injury in mice.

Methods

C57BL/6J mice were subjected to a single thoracic irradiation of 15 Gy X-ray and WP1066 was administrated through intraperitoneal injection. The early and delayed treatment groups were treated with WP1066 during the first 2 weeks and the second 2 weeks, respectively. The therapeutic effects of WP1066 were evaluated by survival analysis, histological examination, and measurement of inflammatory parameters and collagen deposition. The activation of STAT3 pathway was also estimated by immunohistochemical staining and Western blotting.

Results

Delayed treatment of WP1066, but not early treatment, prolonged survival time and prevented the development of radiation pneumonitis and the subsequent lung fibrosis in mice. WP1066 treatment also significantly suppressed the activation of STAT3 signaling in the irradiated lung tissues.

Conclusions

The activation of STAT3 pathway might play an important part in the pathogenesis of radiation-induced lung injury. The protective effects of delayed treatment of WP1066 suggested STAT3 signaling could be a therapeutic target for radiation pneumonitis.

Keywords

WP1066 STAT3 Radiation pneumonitis Inflammation Cytokine 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 81202149), the University Natural Science Research Project of Jiangsu Province (13KJB310016), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

Compliance with Ethical Standards

Conflict of interest

None.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdisciplinary Sciences (RAD-X)Soochow University, Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education InstitutionsSuzhouPeople’s Republic of China

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