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IL-10 and IL-17F Promoter Single Nucleotide Polymorphism and Asthma: A Case-Control Study in South India

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Abstract

Background

Several studies have assessed the association between IL-17F and IL-10 promoter polymorphisms and asthma, but the results were conflicting. Furthermore, few studies have evaluated the association of cytokine polymorphisms with asthma and its clinical phenotypes.

Objective

This study was conducted to evaluate the association of IL-10 (interleukin 10) and IL-17F (interleukin 17F) promoter polymorphisms (rs1800871, rs1800896 and rs1889570) with asthma and its clinical phenotypes including severity, atopic status, spirometric parameters, and response to treatment in south Indian population. A sub-study was conducted to assess cytokine levels in subjects with different gene variants.

Methods

IL-10 and IL-17F polymorphisms were genotyped in 419 asthmatic patients and 393 controls using Mass ARRAY.

Results

Our results showed an association between IL-10 SNPs and mild asthma. No association was found with any of three SNPs in moderate to severe asthma. Comparison of genotype distribution of IL-17F rs1887570 AA variant among atopic and non-atopic patients showed significant difference (p = 0.024). Correlation analysis of IL-10 and IL-17F SNPs to clinical variables showed a positive correlation between IL-17F rs1887570 AA and number of allergen sensitized (rs = 0.142, p = 0.004). Significant improvement in lung function was observed after 2 months of ICS (Inhaled corticosteroids) and LABA (long acting β2 agonist) treatment in all subjects with no statistically significant difference among SNPs variants. Cytokines levels were similar in different SNP variants.

Conclusion

We observed an association between IL-10 rs1800871 and rs1800896 SNPs and mild asthma, as well as IL-17F rs1887570 AA variant and number of allergens sensitized.

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Acknowledgments

The authors thank all the study participants, “Allergy, Asthma, and Chest center” staff and the Chairman, Department of Zoology, University of Mysore.

Conflict of interest

The authors have no conflict of interest to declare.

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Authors and Affiliations

  1. Genetics and Genomics Lab, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, Karnataka, India

    Sareh Raeiszadeh Jahromi, Sangeetha Vishweswaraiah & Nallur. B. Ramachandra

  2. Department of Pulmonary Medicine, J.S.S. Medical College, Mysore, Karnataka, India

    P. A. Mahesh & B. S. Jayaraj

  3. Allergy, Asthma, and Chest Center, Mysore, Karnataka, India

    Amrutha D. Holla

Authors
  1. Sareh Raeiszadeh Jahromi
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  2. P. A. Mahesh
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  3. B. S. Jayaraj
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  4. Amrutha D. Holla
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  5. Sangeetha Vishweswaraiah
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  6. Nallur. B. Ramachandra
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Correspondence to Nallur. B. Ramachandra.

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Raeiszadeh Jahromi, S., Mahesh, P.A., Jayaraj, B.S. et al. IL-10 and IL-17F Promoter Single Nucleotide Polymorphism and Asthma: A Case-Control Study in South India. Lung 193, 739–747 (2015). https://doi.org/10.1007/s00408-015-9753-3

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  • DOI: https://doi.org/10.1007/s00408-015-9753-3

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