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Profibrosing Effect of Angiotensin Converting Enzyme Inhibitors in Human Lung Fibroblasts

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Abstract

Objective

The objective of this study is to determine the effect of two angiotensin-converting enzyme inhibitors (ACEi) (Enalapril and Captopril), an angiotensin-II receptor inhibitor (Losartan) and a renin inhibitor (Aliskiren) on renin, TGF-β1 and collagen expressions in human lung fibroblast cultures through real-time PCR and ELISA.

Materials and Methods

Normal commercial fibroblasts (CCD25) were exposed to 10−6 M of enalapril, captopril, losartan, or aliskiren for 6 h. Subsequently, media were recovered and proteins were concentrated; RNA was extracted from the cells. Real time-PCR and ELISA were performed.

Results

ACEi and losartan-stimulated fibroblasts showed an increase in the expression of TGF-β1, Collagen-Iα1 (Col-Iα1), and renin (except losartan) vs PolR2A (p < 0.05), and upregulation of TGF-β1 protein (p < 0.01), except with aliskiren.

Conclusion

Results show that ACEis and losartan could play a profibrosing role by inducing the overexpression of molecules such TGF-β1 and Collagen.

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Authors and Affiliations

  1. Departamento de Biología Molecular, Instituto Nacional de Enfermedades Respiratorias,”Ismael Cosío Villegas”, Calz. Tlalpan 4502, Col. Sección XVI, 14080, D.F., Mexico

    Gabriela Díaz-Piña, Marco Checa, Carina Becerril, Carolina García de Alba, Anita Vega & Victor Ruiz

  2. Clínica de Asma, Instituto Nacional de Enfermedades Respiratorias, Calz. Tlalpan 4502, Col. Sección XVI, 14080, D.F., Mexico

    Eduardo Montes & Ignacio Páramo

  3. Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Unidad de Investigación Médica en Genética Humana, Av. Cuauhtémoc No. 330, Col. Doctores, 06720, D.F., Mexico

    Rosa Ordoñez-Razo

Authors
  1. Gabriela Díaz-Piña
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  2. Eduardo Montes
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  3. Marco Checa
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  4. Carina Becerril
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  5. Carolina García de Alba
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  6. Anita Vega
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  7. Ignacio Páramo
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  8. Rosa Ordoñez-Razo
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  9. Victor Ruiz
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Corresponding author

Correspondence to Victor Ruiz.

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Díaz-Piña, G., Montes, E., Checa, M. et al. Profibrosing Effect of Angiotensin Converting Enzyme Inhibitors in Human Lung Fibroblasts. Lung 193, 199–202 (2015). https://doi.org/10.1007/s00408-015-9688-8

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  • DOI: https://doi.org/10.1007/s00408-015-9688-8

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