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Recruitment and Phenotypic Characteristics of Interleukin 9-Producing CD4+ T Cells in Malignant Pleural Effusion

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Abstract

Background

Our previous data have demonstrated that the number of IL-9-producing CD4+ T cells (Th9 cells) in malignant pleural effusion (MPE) was significantly increased when compared with that in blood. The aim of the present study was to investigate the mechanism by which Th9 cells were recruited into MPE and the phenotypic characteristics of pleural Th9 cells.

Methods

The expression patterns of chemokine receptors (CCRs) on Th9 cells and the chemoattractant activity of chemokine CCL20 for Th9 cells in vitro were observed. The phenotypic features of Th9 cells in MPE were determined by flow cytometry.

Results

We found that Th9 cells in both MPE and blood expressed a high level of CCR6 on their surface. An in vitro migration assay confirmed that both MPE and supernatants of cultured pleural mesothelial cells could induce the migration of Th9 cells, and anti-CCL20 mAb significantly inhibited the ability of MPE or supernatants to stimulate Th9 cell chemotaxis. We also noted that pleural Th9 cells expressed high levels of CD45RO and very low levels of CD45RA and CD62L, displaying the phenotype of effector memory cells.

Conclusions

Our data revealed that recruitment of Th9 cells into MPE could be induced by pleural CCL20 and that the majority of Th9 cells in MPE displayed the phenotype of effector memory cells.

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Acknowledgments

This work was supported in part by a Grant from National Science Fund for Distinguished Young Scholars of China (No. 30925032), and in part by Grants (No. 81272591 and No. 81270149) from National Natural Science Foundation of China.

Conflict of interest

The authors have no conflicts of interest or financial ties to disclose.

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Authors and Affiliations

  1. Department of Respiratory and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, 8 Gongren Tiyuchang Nanlu, Chaoyang, 100020, Beijing, China

    Xiao-Ning Bu, Zhi-Jian Ye, Zhao-Hui Tong & Huan-Zhong Shi

  2. Department of Respiratory and Critical Care Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

    Qiong Zhou, Jian-Chu Zhang & Zhi-Jian Ye

Authors
  1. Xiao-Ning Bu
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  2. Qiong Zhou
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  3. Jian-Chu Zhang
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  4. Zhi-Jian Ye
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  5. Zhao-Hui Tong
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  6. Huan-Zhong Shi
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Corresponding author

Correspondence to Huan-Zhong Shi.

Additional information

X.-N. Bu and Q. Zhou contributed equally to this work.

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Bu, XN., Zhou, Q., Zhang, JC. et al. Recruitment and Phenotypic Characteristics of Interleukin 9-Producing CD4+ T Cells in Malignant Pleural Effusion. Lung 191, 385–389 (2013). https://doi.org/10.1007/s00408-013-9474-4

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  • DOI: https://doi.org/10.1007/s00408-013-9474-4

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