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Platelet Activating Factor in Surfactant-TA is Inhibited by Coexisting Inhibitors

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Abstract

To clarify whether or not the platelet activating factor (PAF) present in surfactant-TA (calf-lung extract) is harmful, we investigated the activity and inhibitory activity of PAF in a surfactant preparation using a bioassay with washed rabbit platelets. The surfactant-TA contained PAF at 11–21 pmol/vial. The fractions of lysophosphatidylcholine, sphingomyelin, phosphatidylserine plus phosphatidylinositol, and phosphatidylglycerol dose-dependently inhibited the aggregation of washed rabbit platelets induced by PAF. Surfactant-TA contained sufficient amounts of these phospholipids to inhibit the PAF activity completely. These results suggest that coexisting PAF inhibitors protect the lung from the harmful effects of PAF in surfactant-TA.

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Acknowledgements

The author wishes to thank Professor Yunosuke Ogawa, Department of Pediatrics, Saitama Medical Center, Saitama Medical School, for the continuous advice and encouragement throughout this study.

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  1. Department of Pediatrics, Saitama Medical Center, Saitama Medical School, 1981 Kamoda-Tsujido, Kawagoe, Saitama 350-8550, Japan

    N. Koyama

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Koyama, N. Platelet Activating Factor in Surfactant-TA is Inhibited by Coexisting Inhibitors . Lung 181, 213–218 (2003). https://doi.org/10.1007/s00408-003-1023-0

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  • DOI: https://doi.org/10.1007/s00408-003-1023-0

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