Skip to main content

Advertisement

SpringerLink
Log in

Mycobacteria Induces Pleural Mesothelial Permeability by Down-Regulating β-Catenin Expression

  • Published:
Lung Aims and scope Submit manuscript

Abstract

Patients with pulmonary tuberculosis develop pleural effusions with a high protein content. Pleural mesothelial adherens junctions promote mesothelial cell-cell adhesion and contribute to pleural integrity. In the present study we have investigated the effect of mycobacterium (BCG) on mesothelial cell adherens junction proteins and pleural permeability. BCG enhanced pleural mesothelial cell (PMC) release of vascular endothelial growth factor (VEGF), and decreased electrical resistance across the PMC monolayer. Neutralizing antibodies to VEGF significantly restored the drop in PMC electrical resistance caused by BCG. BCG infection down regulated β-catenin (adherens junction protein) expression and caused increased permeability across confluent mesothelial monolayer. Our results suggest that in TB pleurisy, mycobacteria cause VEGF release from mesothelial cells and leads to protein exudation by altering mesothelial adherens junction proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1
Figure 2
Figure 3
Figure 4
Figure 5

Similar content being viewed by others

References

  1. H Aberle H Schwartz R Kemler (1996) ArticleTitleCadherin-catenin complex: protein interactions and their implications for cadherin function. J Cell Biochem 61 IssueID4 514–523 Occurrence Handle1:CAS:528:DyaK28Xkt1Wqu74%3D Occurrence Handle8806074

    CAS  PubMed  Google Scholar 

  2. BR Bloom CJ Murray (1992) ArticleTitleTuberculosis: commentary on a reemergent killer. Science 257 IssueID5073 1055–1064 Occurrence Handle1:STN:280:By2A2snlt1Q%3D Occurrence Handle1509256

    CAS  PubMed  Google Scholar 

  3. RE Bryant CJ Salmon (1996) ArticleTitlePleural empyema. Clin Infect Dis 22 IssueID5 747–762 Occurrence Handle1:STN:280:BymA3cbgtl0%3D Occurrence Handle8722927

    CAS  PubMed  Google Scholar 

  4. PD Collins DT Connolly TJ Williams (1993) ArticleTitleCharacterization of the increase in vascular permeability induced by vascular permeability factor in vivo. Br J Pharmacol 109 IssueID1 195–199 Occurrence Handle1:CAS:528:DyaK3sXkt1Witbo%3D Occurrence Handle7684302

    CAS  PubMed  Google Scholar 

  5. E Dejana G Bazzoni MG Lampugnani (1999) ArticleTitleVascular endothelial (VE)-cadherin: only an intercellular glue? Exp Cell Res 252 IssueID1 13–19 Occurrence Handle1:CAS:528:DyaK1MXmt1Wqtr8%3D Occurrence Handle10502395

    CAS  PubMed  Google Scholar 

  6. N Ferrara T Davis-Smyth (1997) ArticleTitleThe biology of vascular endothelial growth factor. Endocr Rev 18 IssueID1 4–25 Occurrence Handle1:CAS:528:DyaK2sXhsFCiu7s%3D Occurrence Handle9034784

    CAS  PubMed  Google Scholar 

  7. S Kamholz (1996) Pleural tuberculosis. WN Rom SM Garay (Eds) Tuberculosis. Little Brown and Company Boston 483–491

    Google Scholar 

  8. HC Lichtenbeld N Ferarra RK Jain LL Munn (1999) ArticleTitleEffect of local anti-VEGF antibody treatment on tumor microvessel permeability. Microvasc Res 57 IssueID3 357–362 Occurrence Handle10.1006/mvre.1998.2140 Occurrence Handle1:CAS:528:DyaK1MXislOhsbc%3D Occurrence Handle10329263

    Article  CAS  PubMed  Google Scholar 

  9. RW Light (1995) ArticleTitleDiseases of the pleura. Curr Opin Pulm Med 1 IssueID4 313–317 Occurrence Handle1:STN:280:DyaK1c%2FivFemtQ%3D%3D Occurrence Handle9363070

    CAS  PubMed  Google Scholar 

  10. KA Mohammed N Nasreen J Hardwick CS Logie CE Patterson VB Antony (2001) ArticleTitleBacterial induction of pleural mesothelial monolayer barrier dysfunction. Am J Physiol Lung Cell Mol Physiol 281 IssueID1 L119–L125 Occurrence Handle1:CAS:528:DC%2BD3MXltVKgsrw%3D Occurrence Handle11404254

    CAS  PubMed  Google Scholar 

  11. AB Moy J Van Engelenhoven J Bodmer J Kamath C Keese I Giaever S Shasby DM Shasby (1996) ArticleTitleHistamine and thrombin modulate endothelial focal adhesion through centripetal and centrifugal forces. J Clin Invest 97 IssueID4 1020–1027 Occurrence Handle8613524

    PubMed  Google Scholar 

  12. N Nasreen KA Mohammed MJ Ward VB Antony (1999) ArticleTitleMycobacterium-induced transmesothelial migration of monocytes into pleural space: role of intercellular adhesion molecule-1 in tuberculous pleurisy. J Infect Dis 180 IssueID5 1616–1623 Occurrence Handle10.1086/315057 Occurrence Handle1:CAS:528:DyaK1MXnsVKgsLo%3D Occurrence Handle10515824

    Article  CAS  PubMed  Google Scholar 

  13. N Nasreen KA Mohammed G Galffy MJ Ward VB Antony (2000) ArticleTitleMCP-1 in pleural injury: CCR2 mediates haptotaxis of pleural mesothelial cells. Am J Physiol Lung Cell Mol Physiol 278 IssueID3 L591–L598 Occurrence Handle1:CAS:528:DC%2BD3cXhvFSjsLg%3D Occurrence Handle10710532

    CAS  PubMed  Google Scholar 

  14. CL Nilsson M Puchades A Westman K Blennow P Davidsson (1999) ArticleTitleIdentification of proteins in a human pleural exudate using two-dimensional preparative liquid-phase electrophoresis and matrix-assisted laser desorption/ionization mass spectrometry. Electrophoresis 20 IssueID4–5 860–865 Occurrence Handle10.1002/(SICI)1522-2683(19990101)20:4/5<860::AID-ELPS860>3.0.CO;2-I Occurrence Handle1:CAS:528:DyaK1MXjsVakt74%3D Occurrence Handle10344259

    Article  CAS  PubMed  Google Scholar 

  15. WG Roberts GE Palade (1995) ArticleTitleIncreased microvascular permeability and endothelial fenestration induced by vascular endothelial growth factor. J Cell Sci 108 IssueID6 2369–2379 Occurrence Handle1:CAS:528:DyaK2MXmsFOnsrs%3D Occurrence Handle7673356

    CAS  PubMed  Google Scholar 

  16. H Roh DW Green CB Boswell JA Pippin JA Drebin (2001) ArticleTitleSuppression of beta-catenin inhibits the neoplastic growth of APC-mutant colon cancer cells. Cancer Res 61 IssueID17 6563–6568 Occurrence Handle1:CAS:528:DC%2BD3MXmsF2jtrk%3D Occurrence Handle11522655

    CAS  PubMed  Google Scholar 

  17. M Takeichi (1990) ArticleTitleCadherins: a molecular family important in selective cell-cell adhesion. Annu Rev Biochem 59 237–252 Occurrence Handle1:CAS:528:DyaK3cXls1Ontr8%3D Occurrence Handle2197976

    CAS  PubMed  Google Scholar 

  18. M Takeichi (1995) ArticleTitleMorphogenetic roles of classic cadherins. Curr Opin Cell Biol 7 IssueID5 619–627 Occurrence Handle1:CAS:528:DyaK2MXosVKnsr8%3D Occurrence Handle8573335

    CAS  PubMed  Google Scholar 

  19. Tuberculosis. WHO Bulletin. Fact Sheet No. 104 (Revised) http://www.who.int/mediacentre/factsheets/who104/en/

  20. GP van Nieuw Amerongen R Draijer MA Vermeer VW van Hinsbergh (1998) ArticleTitleTransient and prolonged increase in endothelial permeability induced by histamine and thrombin: role of protein kinases, calcium, and RhoA. Circ Res 83 IssueID11 1115–1123 Occurrence Handle9831706

    PubMed  Google Scholar 

  21. N-S Wang (1985) Mesothelial cell in situ. The pleura in health and disease, vol. 30 Marcel Dekker Inc. New York 23–42

    Google Scholar 

  22. PM Wang SJ Lai-Fook (1999) ArticleTitleRegional pleural filtration and absorption measured by fluorescent tracers in rabbits. Lung 177 IssueID5 289–309 Occurrence Handle10.1016/S0378-1097(99)00346-8 Occurrence Handle1:CAS:528:DyaK1MXkvVCjsr8%3D Occurrence Handle10467021

    Article  CAS  PubMed  Google Scholar 

  23. AS Yap WM Brieher BM Gumbiner (1997) ArticleTitleMolecular and functional analysis of cadherin-based adherens junctions. Annu Rev Cell Dev Biol 13 119–146 Occurrence Handle1:CAS:528:DyaK1cXisFSksw%3D%3D Occurrence Handle9442870

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Veterans' Affairs Medical Center, Indiana University School of Medicine, 1481 West 10th Street, 111-P, Indianapolis, IN 46202, USA

    K. A. Mohammed, N. Nasreen, J. Hardwick, R. D. Van Horn, K. L. Sanders & V. B. Antony

Authors
  1. K. A. Mohammed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. Nasreen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Hardwick
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. D. Van Horn
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. L. Sanders
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. V. B. Antony
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. B. Antony.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mohammed, K., Nasreen, N., Hardwick, J. et al. Mycobacteria Induces Pleural Mesothelial Permeability by Down-Regulating β-Catenin Expression . Lung 181, 57–66 (2003). https://doi.org/10.1007/s00408-003-1006-1

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00408-003-1006-1

Keywords

Search

Navigation