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Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Objectives

TAM receptors (TYRO3, AXL, and MER) play important roles in inflammatory responses, but their effects in chronic rhinosinusitis with nasal polyps (CRSwNP) remain elucidated. We aim to evaluate the values of TAM receptors in disease severity and postoperative recurrence of CRSwNP.

Methods

We initially enrolled 160 patients with CRSwNP who were treated with functional endoscopic sinus surgery (FESS) and postoperative recurrence was evaluated during the follow-up period. Circulating TAM receptor levels were detected by enzyme-linked immunosorbent assay (ELISA), and tissue expressions were measured by real-time polymerase chain reaction (RT-PCR) and immunohistochemical (IHC). The relationships between TAM receptor levels and postoperative recurrence were examined.

Results

A total of 150 patients completed the follow-up schedule, 49 patients experienced postoperative recurrence and the remaining 101 patients were non-recurrent. In recurrent CRSwNP patients, serum levels of TAM receptors were increased compared to those in non-recurrent patients and were positively correlated with disease severity scores (P < 0.05). Circulating TYRO3 and MER were identified as potential predictors of postoperative recurrence based on receiver operating characteristics (ROC) and Kaplan–Meier plots (P < 0.05). Furthermore, tissue TAM receptor levels, as determined by both RT-PCR and IHC, were enhanced in the recurrent group than in the non-recurrent group (P < 0.05) and were predictive of postoperative recurrence (P < 0.05). Interestingly, circulating TYRO3 and MER concentrations, as well as tissue TYRO3 expression, were found to be significantly increased in patients who experienced postoperative recurrence (P < 0.05). IHC images from the same patients revealed that TAM expressions were enhanced in the recurrent tissues compared to their baseline tissue levels.

Conclusions

Our laboratory results demonstrated that TAM receptors were increased in recurrent CRSwNP patients and associated with postoperative recurrence. Moreover, the new laboratory findings suggested that measuring circulating levels of TAM receptors might serve as a promising new approach to assess disease progression and predict the risk of postoperative recurrence.

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Funding

This research was supported by the Natural Science Foundation of Hunan Province (2021JJ30754) and the Hunan Provincial Health Commission Scientific Research Project (202107010164).

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Authors and Affiliations

  1. Changsha Aier Eye Hospital, Aier Eye Hospital Group, Changsha, Hunan, China

    Yandan Zhou

  2. Department of Otolaryngology Head and Neck Surgery, The Affiliated Changsha Central Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, China

    Zhili Feng, Jie Wen, Chi Yang & Qiancheng Jing

  3. Institute of Otolaryngology Head and Neck Surgery, Hengyang Medical School, University of South China, Changsha, Hunan, China

    Zhili Feng, Jie Wen, Chi Yang & Qiancheng Jing

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Correspondence to Qiancheng Jing.

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This study was conducted following the recommendations of the Declaration of Helsinki. The Human Ethical Committee of the Affiliated Changsha Central Hospital, Hengyang Medical School of the University of South China approved this study (protocol: 2023005). Informed consent was obtained from the study participants before the study commencement.

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405_2024_8450_MOESM4_ESM.tif

Figure S1 The tissue mRNA levels of TAM receptors in CRSwNP patients without AR/AS. (A–C) When removed the patients with AR or AS, the tissue TAM receptor mRNA expressions were elevated in the recurrence group in comparison with the non-recurrence group. *P<0.05, ***P<0.001. CRSwNP, chronic rhinosinusitis with nasal polyps; AR, allergic rhinitis; AS, asthma

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Zhou, Y., Feng, Z., Wen, J. et al. Aberrant expressions of TAM receptors are associated with postoperative recurrence in chronic rhinosinusitis with nasal polyps. Eur Arch Otorhinolaryngol 281, 3005–3015 (2024). https://doi.org/10.1007/s00405-024-08450-1

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