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Ferroptosis-related genes for predicting prognosis of patients with laryngeal squamous cell carcinoma

  • Laryngology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Background

Previous studies reported that ferroptosis-related genes can regulate the process of tumor cell changes by regulating iron metabolism. However, the prognostic value of ferroptosis-related genes in LC remains to be further elucidated.

Methods

Ferroptosis-related gene expression profiles of coexisting ferroptosis-related genes were extracted from both cohorts (TCGA and GSE27020) for eligible analysis. LASSO Cox regression was utilized to build an optimum ferroptosis-related prognostic model. Kaplan–Meier curve was performed by log‐rank test, and time‐dependent ROC curve was constructed to evaluate the predictive power of this signature in both cohorts. GO and KEGG enrichment analysis was used to investigate the potential mechanism of differential enrichment signal pathways.

Results

112 LC patients from the TCGA cohort and 108 LC patients with clinical information from the GEO cohorts were eventually included in the study. Three ferroptosis-related genes were identified as an independent risk factor to establish the prognostic risk score. Kaplan–Meier curve represented that patients with high-risk group favors with worse OS than their low-risk group (P = 0.04). The good performance of the gene signature for predicting OS was evaluated by area under the curve (AUC) of time-dependent ROC curves achieved 0.74 at 3 years, and 0.70 at 5 years. Similar performance has been proved in the external validation cohort. GO and KEGG enrichment analysis have been performed to explore the signaling pathways and underlying mechanisms were significantly active in LC patients.

Conclusion

In summary, our study developed a ferroptosis-related model that could be an effective biomarker to predict the prognosis of laryngeal cancer.

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Data availability

The data used to support the findings of this study are included within the article.

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Acknowledgements

The authors thank the efforts of the National Cancer Institute in the creation of the TCGA and GEO databases.

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Author notes

  1. Wenfei Li and Fang Han have contributed equally to this work.

Authors and Affiliations

  1. Department of Radiology, Affiliated Zhongshan Hospital of DaLian University, Dalian, Liaoning, China

    Fang Han & Yutong Yao

  2. Department of Radiology, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei, China

    Wenfei Li, Jinglong Li & Zhanqiu Wang

  3. Department of PET-CT, Xiangyang Central Hospital, Xiangyang, Hubei, China

    Tao Chen

  4. Department of Endocrine, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei, China

    Di Wang

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  1. Fang Han
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Contributions

FH and WF conceived and designed the study; FH and WF collected the data. WF and TC analyzed the data. ZW and DW contributed to the writing and revision of the manuscript. YY and JL participate in external data collection and verification.

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Correspondence to Fang Han.

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Supplementary Information

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405_2021_6789_MOESM1_ESM.rar

Supplementary file1 Fig. S1 a, b Prognostic value detection of the gene signature and clinical features via univariate (green) and multivariate (red) Cox regression analysis in TCGA cohort. c, d Prognostic value detection of the gene signature and clinical features via univariate (green) and multivariate (red) Cox regression analysis in GEO cohort. The results identified that our signature risk score was an independent prognostic factor in training and validation cohorts. Fig. S2 a Ferroptosis-related gene signature in TCGA cohort. b, c. The distribution and survival status of patients with risk score in the laryngeal cancer in TCGA cohort. d Ferroptosis-related gene signature in GEO cohort. e, f. The distribution and survival status of patients with risk score in the laryngeal cancer in GEO cohort. (RAR 1231 KB)

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Han, F., Li, W., Chen, T. et al. Ferroptosis-related genes for predicting prognosis of patients with laryngeal squamous cell carcinoma. Eur Arch Otorhinolaryngol 278, 2919–2925 (2021). https://doi.org/10.1007/s00405-021-06789-3

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