Abstract
Background
Vocal cord scarring is the most crucial obstacle in voice quality after surgery. This study aimed to evaluate the effectiveness of hyaluronic acid (HA)-collagen nanofibers on the healing of vocal cords after surgical trauma.
Method
Right vocal cords of 12 New Zealand white rabbits were traumatized, and the experimental group was received 1.08 mg/75 ml topical HA-collagen nanofiber (Gelfix® spray) for 3 days. Three animals in each group were sacrificed on the 7th day, and the remaining of the animals were sacrificed on the 21st day. The laryngeal specimens in the experimental and control groups were examined histopathologically.
Result
The 7th-day H&E staining evaluation revealed pink, dense, and thin collagen fibers. Besides, the collagen content was scattered and irregular in the experimental group. The 21st-day assessment showed that the collagen bundles in the granulation tissue were almost with the same formation in both of the groups. Masson Trichrome staining on the 7th day of the study showed that the collagen fiber bundles were less frequent in the control group than the experimental group. The 7th-day Van Gieson staining analysis showed that the pattern of reticular fibers was more regular with the parallel formation in the experimental group than the control group.
Conclusion
HA-collagen nanofiber can be used in diseases that impair voice quality due to the thickening of the lamina propria layer in the vocal cord and impaired viscoelasticity due to fibrosis after tissue trauma.
Similar content being viewed by others
References
Kieff DA, Zeitels SM (1996) Phonosurgery. Compr Ther 22(4):222–230
Friedrich G, Dikkers FG, Arens C, Remacle M, Hess M, Giovanni A, Duflo S, Hantzakos A, Bachy V, Gugatschka M (2013) European Laryngological Society. Phonosurgery Committee. Vocal fold scars: current concepts and future directions. Consensus report of the Phonosurgery Committee of the European Laryngological Society. Eur Arch Otorhinolaryngol 270(9):2491–2507
Hull JH, Backer V, Gibson PG, Fowler SJ (2016) Laryngeal dysfunction: assessment and management for the clinician. Am J Respir Crit Care Med 194(9):1062–1072
Hirano S, Burly M, Fase T (2009) Histologic characterization of human scarred vocal folds. J Voice Off J Voice Found 23(4):399–407
Arens C, Voigt-Zimmermann S (2016) Chirurgie des larynx—Teil 1: benigne läsionen. Laryngorhinootologie 95(6):419–436 (German)
Bless DM, Welham NV (2010) Characterization of vocal fold scar formation, prophylaxis, and treatment using animal models. Curr Opin Otolaryngol Head Neck Surg 18(6):481–486
Mattei A, Magalon J, Bertrand B, Philandrianos C, Veran J, Giovanni A (2017) Cell therapy and vocal fold scarring. Eur Ann Otorhinolaryngol Head Neck Dis 134(5):339–345
Truzzi GM, Pauna HF, Bette P, Gusmão RJ, Crespo AN, Semenzati GO (2017) Methods of fat tissue processing for human vocal fold injection: a systematic review. J Voice 31(2):244.e17-244.e21
Brandenburg JH, Kirkham W, Koschkee D (1992) Vocal cord augmentation with autogenous fat. Laryngoscope 102(5):495–500
Veves A, Sheehan P, Pham HT (2002) A randomized, controlled trial of Promogran (a collagen/oxidized regenerated cellulose dressing) vs standard treatment in the management of diabetic foot ulcers. Arch Surg 137(7):822–827
Raghavendra R, Reddy Y, Mer F (2012) Role of collagen impregnated with dexamethasone and placentrix in patients with oral submucous fibrosis. J Maxillofac Oral Surg 11(2):166–170
Walimbe T, Panitch A, Sivasankar PM (2017) A Review of hyaluronic acid and hyaluronic acid-based hydrogels for vocal fold tissue engineering. J Voice 231(4):416–423
Kanemaru S, Lyu T, Gery M (2003) Regeneration of the vocal fold using autologous mesenchymal stem cells. Ann Otol Rhinol Laryngol 112(11):915–920
Rousseau B, Messen R, Hyu R (2006) Investigation of anti-hyaluronidase treatment on vocal fold wound healing. J Voice Off J Voice Found 20(3):443–451
Garrett CG, Per S, Tred Y (2001) Effect of mitomycin-C on vocal fold healing in a canine model. Ann Otol Rhinol Laryngol 110(1):25–30
Akdogan O, Selcuk A, Ozcan I (2009) Activation of vocal fold healing with topical vitamin A in rabbits. Acta Otolaryngol 129(2):220–224
Campagnolo AM, Beer S, Curry F (2010) Histologic study of acute vocal fold wound healing after corticosteroid injection in a rabbit model. Ann Otol Rhinol Laryngol 119(2):133–139
Gaston J, Sylvia L, Thibeault S (2013) Hyaluronic acid hydrogels for vocal fold wound healing. Biomatter 3(1):e23799
Mohan R, Hunasgi S, Peral F (2011) Significant inhibition of corneal scarring in vivo with tissue-selective, targeted AAV5 decorin gene therapy. Invest Ophthalmol Vis Sci 52(7):4833–4841
Hunasgi S, Vanishree M, Shamala R (2013) Keloid: A case report and review of pathophysiology and differences between keloid and hypertrophic scars. J Oral Maxillofac Pathol 1:116–120
Gilbert TW, Gilbert MR, Povirk KM (2009) Liver-derived extracellular matrix as a biologic scaffold for acute vocal fold repair in a canine model. Laryngoscope 119:1856–1863
Manizate F, Kim HT, Kuo AC (2012) A prospective, single-center, nonblinded, comparative, postmarket clinical evaluation of a bovine-derived collagen with ionic silver dressing versus a carboxymethylcellulose and ionic silver dressing for the reduction of bioburden in variable-etiology, bilateral lower-extremity wounds. Adv Skin Wound Care 25(5):220–225
Entekhabi E, Haghbin Nazarpak M, Shafieian M, Mohammadi H, Firouzi M, Hassannejad Z (2021) Fabrication and in vitro evaluation of 3D composite scaffold based on collagen/hyaluronic acid sponge and electrospun polycaprolactone nanofibers for peripheral nerve regeneration. J Biomed Mater Res A 109(3):300–312
Li L, Stiadle JM, Lau HK, Zerdoum AB, Jia X, Thibeault SL, Kiick KL (2016) Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration. Biomaterials 108:91–110
Kirsch A, Hortobagyi D, Stachl T, Karbiener M, Grossmann T, Gerstenberger C, Gugatschka M (2019) Development and validation of a novel phonomimetic bioreactor. PLoS ONE 14(3):e0213788
Gugatschka M, Ohno S, Saxena A, Hirano S (2012) Regenerative medicine of the larynx. Where are we today? a review. J Voice 26(5):670.e7–13
Kim YM, Oh SH, Choi JS, Lee S, Ra JC, Lee JH, Lim J (2014) Adipose-derived stem cell-containing hyaluronic acid/alginate hydrogel improves vocal fold wound healing. Laryngoscope 124:64–72
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Elibol, E., Yılmaz, Y.F., Ünal, A. et al. Effects of hyaluronic acid-collagen nanofibers on early wound healing in vocal cord trauma. Eur Arch Otorhinolaryngol 278, 1537–1544 (2021). https://doi.org/10.1007/s00405-021-06703-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00405-021-06703-x