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Platelet-rich plasma for laryngotracheal reconstruction: an experimental study

  • Laryngology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Objectives/hypothesis

This study was designed to evaluate the graft healing effect of topical application of platelet-rich plasma (PRP) for laryngotracheal reconstruction (LTR) in a rabbit model.

Study design

It is a prospective randomized control animal study.

Materials and methods

Sixteen healthy New Zealand White rabbits were assigned to two groups of eight animals each. The control group underwent LTR with anterior auricular cartilage graft. The PRP group underwent the same surgical procedure plus PRP application over the anastomosis and surgical field. Two animals in the PRP group and two animals in control group died due to severe respiratory distress on postoperative days 10, 12, 15, and 18. Six rabbits (n = 3 for control group and n = 3 for PRP group) were sacrificed at 4 weeks, and six rabbits (n = 3 for control group and n = 3 for PRP group) were sacrificed at 8 weeks. Laryngotracheal regions were evaluated histopathologically.

Results

Macroscopically, the average anteroposterior and lateral diameter of the reconstructed region and the degree of lumen patency on postoperative 4th week and 8th week were not statistically different among two groups. There was no significant difference between the groups in terms of any of the microscopic findings when the analysis was made separately. However, analysis of the total number of rabbits has shown that new cartilage formation and angiogenesis were more pronounced in PRP group than control group.

Conclusions

Application of PRP contributed to better healing in airway surgery by promoting a release of growth factors that stimulate new cartilage formation and angiogenesis.

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Acknowledgements

This research was supported by Science Research Program of Health Sciences University.

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Authors and Affiliations

  1. Department of Otolaryngology, Umraniye Research and Education Hospital, University of Health Sciences, Elmalıkent Mahallesi Adem Yavuz Caddesi, Umraniye, 34764, Istanbul, Turkey

    Serap Sahin Onder, Asli Sahin Yilmaz & Burak Erkmen

  2. Department of Pathology and Molecular Medicine, Umraniye Research and Education Hospital, University of Health Sciences, Istanbul, Turkey

    Cumhur Selçuk Topal

  3. Department of Otolaryngology, Medipol International Health Center Camlıca Hospital, Istanbul, Turkey

    Ozgul Gergin

  4. Department of Otolaryngology, Uskudar Hospitalturk Hospital, Istanbul, Turkey

    Mustafa Salih Canpolat

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  1. Serap Sahin Onder
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  2. Asli Sahin Yilmaz
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Correspondence to Serap Sahin Onder.

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Sahin Onder, S., Sahin Yilmaz, A., Erkmen, B. et al. Platelet-rich plasma for laryngotracheal reconstruction: an experimental study. Eur Arch Otorhinolaryngol 277, 3103–3109 (2020). https://doi.org/10.1007/s00405-020-06091-8

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  • DOI: https://doi.org/10.1007/s00405-020-06091-8

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