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Expression of Cl− channels/transporters in nasal polyps

  • Rhinology
  • Published:
European Archives of Oto-Rhino-Laryngology Aims and scope Submit manuscript

Abstract

Purpose

Nasal polyp formation is a common sequela of prolonged chronic rhinosinusitis, but the mechanism underlying this disease state is still controversial. We compared the expressions of Cl− channels/transporters in nasal polyps with those in inferior turbinates to explore whether a deficiency in Cl− transport may participate in the pathophysiology of nasal polyp formation as in patients with cystic fibrosis.

Methods

Nasal polyps and inferior turbinates were collected from 12 chronic rhinosinusitis patients with hypertrophic rhinitis and/or nasal polyps. Expressions of cystic fibrosis transmembrane conductance regulator (CFTR), pendrin, Na+–K+–2Cl− cotransporter 1 (NKCC1), SLC26A3, TMEM16A and anion exchanger 2 (AE2) were examined by fluorescence immunohistochemistry using Alexa Fluor 488.

Results

CFTR was weakly expressed on the epithelial surface of the turbinate mucosa whereas the nasal polyps showed almost no fluorescence. Pendrin was mainly expressed on the epithelial surface in both tissues. The fluorescence was moderate in the nasal polyps and strong in the turbinate mucosa. For NKCC1, moderate fluorescence was observed throughout the entire epithelial layer of the nasal polyps, but the turbinate mucosa exhibited almost no fluorescence. On the other hand, no fluorescence for SLC26A3, TMEM16A or AE2 was seen in either tissue.

Conclusion

These results suggest that CFTR, pendrin and NKCC1 may participate in the pathogenesis of nasal mucosal edema and play roles in the mechanism of nasal polyp formation.

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Funding

This study was supported by a Grant-in-Aid for Scientific Research (C) (no. 19K09879; 2019–2022) to H. S. from the Japan Society for the Promotion of Science.

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Author notes

  1. Thi Nga Nguyen and Hideaki Suzuki contributed equally to this work.

Authors and Affiliations

  1. Department of Otorhinolaryngology-Head and Neck Surgery, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu, Fukuoka, 807-8555, Japan

    Thi Nga Nguyen, Ba Hung Do, Takuro Kitamura, Jun-ichi Ohkubo, Tetsuro Wakasugi, Toyoaki Ohbuchi & Hideaki Suzuki

Authors
  1. Thi Nga Nguyen
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  2. Ba Hung Do
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  3. Takuro Kitamura
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  4. Jun-ichi Ohkubo
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  5. Tetsuro Wakasugi
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  6. Toyoaki Ohbuchi
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  7. Hideaki Suzuki
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Corresponding author

Correspondence to Hideaki Suzuki.

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The authors have no other funding, financial relationships or conflicts of interest to disclose.

Ethical approval

All procedures performed in the study were approved by the institutional review board of the University of Occupational and Environmental Health.

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Informed consent was obtained from all individual participants included in the study.

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Nguyen, T.N., Do, B.H., Kitamura, T. et al. Expression of Cl− channels/transporters in nasal polyps. Eur Arch Otorhinolaryngol 277, 2263–2270 (2020). https://doi.org/10.1007/s00405-020-05981-1

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  • DOI: https://doi.org/10.1007/s00405-020-05981-1

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