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G protein coupled oestrogen receptor 1, aromatase, 17β-HSD and cAMP level in mutational falsetto

  • Laryngology
  • Published:
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Abstract

Objectives

Puberphonia or mutational falsetto (MF) is seen more in males, and hormonal changes are considered to be among the aetiological causes. Therefore, the aim of this study was to investigate the molecules [G protein-coupled oestrogen receptor 1 (GPER-1), aromatase, 17-beta-hydroxysteroid dehydrogenase (17β-HSD), cyclic adenosine monophosphate (cAMP) levels] related to receptors and pathways in patients with MF.

Methods

The study included 30 MF patients and a control group of 30 healthy individuals. Voice recordings were made of the MF patients and acoustic analyses were applied. The serum GPER-1, aromatase, 17β-HSD, cAMP levels and TSH, estradiol, prolactin, progesterone, and testosterone levels were evaluated in venous blood samples.

Results

In the MF patients, the GPER-1 level determined of mean 3.68 (1.95–4.26) pg/ml, 17 beta dehydrogenase of 5.25 (2.73–6.77) ng/ml, and cAMP of 24.62 (11.62–30.35) ng/ml were statistically signficantly higher than those of the control group (p = 0.008, p = 0.002, p = 0.003, respectively). The aromatase level in the MF patients was found to be 3.48 (2.01–4.91) and the difference between the two groups was not statistically significant (p = 0.067).

Conclusion

The GPER-1, 17β-HSD, and cAMP levels were found to be higher in the MF patients than in the control group, suggesting that they could be of importance in the diagnosis and treatment of MF.

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References

  1. Hammarberg B (1987) Pitch and quality characteristics of mutational voice disorders before and after therapy. Folia Phoniatr (Basel) 39:204–216

    Article  CAS  Google Scholar 

  2. Hacki T, Heitmüller S (1999) Development of the child's voice: premutation, mutation. Int J Pediatr Otorhinolaryngol 49:141–144

    Article  Google Scholar 

  3. Filardo EJ, Quinn JA, Bland KI, Frackelton AR Jr (2000) Estrogen-induced activation of Erk-1 and Erk-2 requires the G protein-coupled receptor homolog, GPR30, and occurs via trans-activation of the epidermal growth factor receptor through release of HB–EGF. Mol Endocrinol 14:1649–1660

    Article  CAS  Google Scholar 

  4. Revankar CM, Cimino DF, Sklar LA, Arterburn JB, Prossnitz ER (2005) A transmembrane intracellular estrogen receptor mediates rapid cell signaling. Science 307:1625–1630

    Article  CAS  Google Scholar 

  5. Filardo EJ, Quinn JA, Frackelton AR Jr, Bland KI (2002) Estrogen action via the G protein-coupled receptor, GPR30: stimulation of adenylyl cyclase and cAMP-mediated attenuation of the epidermal growth factor receptor-to-MAPK signaling axis. Mol Endocrinol 16:70–84

    Article  CAS  Google Scholar 

  6. Beaudoin C, Bonenfant M, Tremblay Y (1997) Regulation of cytochrome P450 cholesterol side-chain cleavage, 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4 isomerase type 1 and estradiol-17 beta-hydroxysteroid dehydrogenase mRNA levels by calcium in human choriocarcinoma JEG-3 cells. Mol Cell Endocrinol 133:63–71

    Article  CAS  Google Scholar 

  7. Carreau S, Bourguiba S, Lambard S et al (2002) Reproductive system: aromatase and estrogens. Mol Cell Endocrinol 193:137–143

    Article  CAS  Google Scholar 

  8. Carreau S, de Vienne C, Galeraud-Denis I (2008) Aromatase and estrogens in man reproduction: a review and latest advances. Adv Med Sci 53:139–144

    Article  CAS  Google Scholar 

  9. Carpino A, Romeo F, Rago V (2004) Aromatase immunolocalization in human ductuli efferentes and proximal ductus epididymis. J Anat 204:217–220

    Article  CAS  Google Scholar 

  10. Pereyra-Martinez AC, Roselli CE, Stadelman HL, Resko JA (2001) Cytochrome P450 aromatase in testis and epididymis of male rhesus monkeys. Endocrine 16:15–19

    Article  CAS  Google Scholar 

  11. Kilic MA, Okur E, Yildirim I et al (2008) Turkish version of the voice handicap index. Kulak Burun Bogaz Ihtis Derg 18:139–147

    PubMed  Google Scholar 

  12. Garcia-Segura LM, Balthazart J (2009) Steroids and neuroprotection: new advances. Front Neuroendocrinol 30(2):v–i. https://doi.org/10.1016/j.yfrne.2009.04.006

    Article  PubMed  PubMed Central  Google Scholar 

  13. Findikli E, Kurutas EB, Camkurt MA et al (2017) Increased serum G protein-coupled estrogen receptor 1 levels and its diagnostic value in drug naïve patients with major depressive disorder. Clin Psychopharmacol Neurosci 15:337–342

    Article  CAS  Google Scholar 

  14. Orhan FÖ, Kurutaş EB, Doğaner A et al (2018) Serum levels of GPER-1 in euthymic bipolar patients. Neuropsychiatr Dis Treat 26:855–862

    Article  Google Scholar 

  15. Bilal N, Kurutas EB, Orhan I (2018) An evaluation of G-protein coupled membrane estrogen receptor-1 level in stuttering. Eur Arch Otorhinolaryngol 275:469–476

    Article  Google Scholar 

  16. Colton RY, Casper JK, Leonard R. Undestanding voice problems. puberfonia. In: Differantial diagnosis of voice problems. 4thEd. Williams and Wilkins, Baltimore, 2011, pp 11–62.

  17. Patel VH, Chen J, Ramanjaneya M et al (2010) G-protein coupled estrogen receptor 1 expression in rat and human heart: protective role during ischaemic stress. Int J Mol Med 26:193–199

    CAS  PubMed  Google Scholar 

  18. Recchia AG, De Francesco EM, Vivacqua A et al (2011) The G protein-coupled receptor 30 is up-regulated by hypoxia-inducible factor-1alpha (HIF-1alpha) in breast cancer cells and cardiomyocytes. J Biol Chem 286:10773–10782

    Article  CAS  Google Scholar 

  19. Martensson UE, Salehi SA, Windahl S et al (2009) Deletion of the G protein-coupled receptor 30 impairs glucose tolerance, reduces bone growth, increases blood pressure, and eliminates estradiol-stimulated insulin release in female mice. Endocrinology 150:687–698

    Article  Google Scholar 

  20. Filardo EJ, Graeber CT, Quinn JA et al (2006) Distribution of GPR30, a seven membrane-spanning estrogen receptor, in primary breast cancer and its association with clinicopathologic determinants of tumor progression. Clin Cancer Res 12:6359–6366

    Article  CAS  Google Scholar 

  21. Smith HO, Leslie KK, Singh M et al (2007) GPR30: a novel indicator of poor survival for endometrial carcinoma. Am J Obstet Gynecol 196:386.e1–11

    Article  Google Scholar 

  22. Smith HO, Arias-Pulido H, Kuo DY et al (2009) GPR30 predicts poor survival for ovarian cancer. Gynecol Oncol 114:465–471

    Article  CAS  Google Scholar 

  23. McNamara KM, Sasano H (2018) The role of 17βHSDs in breast tissue and breast cancers. Mol Cell Endocrinol S0303–7207:30314–30319

    Google Scholar 

  24. Sanderson JT (2006) The steroid hormone biosynthesis pathway as a target for endocrine-disrupting chemicals. Toxicol Sci 94:3–21

    Article  CAS  Google Scholar 

  25. Qu JH, Hong X, Chen JF et al (2008) Fenvalerate inhibits progesterone production through cAMP-dependent signal pathway. Toxicol Lett 176:31–39

    Article  CAS  Google Scholar 

  26. Kim Y, Ryu JC, Choi HS, Lee K (2011) Effect of 2,2′,4,4′-tetra hydroxy benzophenone (BP2) on steroidogenesis in testicular Leydig cells. Toxicology 288:18–26

    Article  CAS  Google Scholar 

  27. Ronco AM, Valdes K, Marcus D, Llanos M (2001) The mechanism for lindane-induced inhibition of steroidogenesis in cultured rat Leydig cells. Toxicology 159:99–106

    Article  CAS  Google Scholar 

  28. Fuchs M, Fröehlich M, Bettina Hentschel B, Eberhard Kruse E, Knauft D (2007) Predicting mutational change in the speaking voice of boys. J Voice 21:169–178

    Article  Google Scholar 

  29. Behlau M, Azevedo R, Pontes P (2001) Desenvolvimento da laringe. In: Behlau M (ed) Voz: o livro do especialista, vol 1. Revinter, Rio de Janeiro, pp 53–56

    Google Scholar 

  30. Laukkanen A-M, Titze IR, Finnegan EM, Hoffman H (2002) Laryngeal muscle activity in a tonal scale: comparing speech-like to song-like productions in a mezzo-soprano. J Singing 59:49–56

    Google Scholar 

  31. Kitajima Y, Ono Y (2016) Estrogens maintain skeletal muscle and satellite cell functions. J Endocrinol 229:267–275

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was presented in 11th National Laryngology Congress.

Funding

This study was supported financially by the Kahramanmaras Sutcu Imam University Scientific research Project unit (Project no. 2018/2-47 M).

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Authors and Affiliations

  1. Department of Otorhinolaringology, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Avsar Campus, 46100, Kahramanmaras, Turkey

    Saime Sagiroglu, Nagihan Bilal & Israfil Orhan

  2. Department of Medical Biochemistry, Faculty of Medicine, Kahramanmaras Sutcu Imam University, Kahramanmaras, Turkey

    Metin Kılınc

  3. Department of Biostatistics and Medical Informatics, Faculty of Medicine, Kahramanmaraş Sutcu Imam University, Kahramanmaras, Turkey

    Adem Doganer

  4. Department of Otorhinolaringology, Faculty of Medicine, Medeniyet University, Istanbul, Turkey

    Mehmet Akif Kılıc

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  1. Saime Sagiroglu
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Correspondence to Saime Sagiroglu.

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Sagiroglu, S., Kılınc, M., Doganer, A. et al. G protein coupled oestrogen receptor 1, aromatase, 17β-HSD and cAMP level in mutational falsetto. Eur Arch Otorhinolaryngol 277, 1121–1127 (2020). https://doi.org/10.1007/s00405-020-05795-1

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